Study of regulatory mechanism for intracellular transport using high efficient genome editing mediated by improved TALEN.

• Project/Area Number: 26440108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ikeda Kazuho 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (20642565)
• Co-Investigator(Renkei-kenkyūsha): Okada Yasushi 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (50272430)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞内物質輸送 / ゲノム編集 / TALEN / 黒色素胞 / メラノソーム / キネシン

Outline of Final Research Achievements

Intracellular transport is essential for numerous cellar functions. Many vesicles and organelles are transported along microtubules bidirectionally by opposing MT motors such as dynein and kinesin. However, regulatory mechanism for bidirectional movement of cargos largely remains unclear. In this study, we investigate tug-of-war theory by reconstructing tug-of-war between opposing MT motors in Xenopus melanophore. We also showed MAP4 mediated transport regulation.

Research Products

• [Int'l Joint Research] UCONN Health Center(米国)
• [Int'l Joint Research] UCONN Health Center(米国)
• [Journal Article] Engineered Tug-of-War Between Kinesin and Dynein Controls Direction of Microtubule Based Transport In Vivo (2016)
  • Author(s): Rezaul K, Gupta D, Semenova I, Ikeda K, Kraikivski P, Yu J, Cowan A, Zaliapin I, Rodionov V.
  • Journal Title: Traffic Volume: 17(5) Issue: 5 Pages: 475-486
  • DOI: 10.1111/tra.12385
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regulation of microtubule-based transport by MAP4. (2014)
  • Author(s): Semenova I, Ikeda K, Resaul K, Kraikivski P, Aguiar M, Gygi S, Zaliapin I, Cowan A, Rodionov V.
  • Journal Title: Molecular Biology of the Cell Volume: 25 Issue: 20 Pages: 3119-32
  • DOI: 10.1091/mbc.e14-01-0022
  • Peer Reviewed / Open Access
• [Presentation] In vitro analysis of super-active TALE protein (2016)
  • Author(s): Ikeda, K., Terahara, Y., Miyashita, N., and Okada, Y.
  • Organizer: ASCB annual meeting
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2016-12-02
  • Int'l Joint Research
• [Presentation] ゲノム任意配列可視化のための高活性TALEプローブの開発 (2016)
  • Author(s): 池田一穂、寺原陽子、隅山健太、岡田康志
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま(郡山市福島県)
  • Year and Date: 2016-03-29
• [Presentation] Development of super-active TALEs for efficient genome visualizing and manipulating tools (2015)
  • Author(s): Kazuho Ikeda, Yoko Terahara, Kenta Sumiyama, Naoyuki Miyashita, and Yasushi Okada
  • Organizer: The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2015-12-19
  • Int'l Joint Research
• [Presentation] In vitro analysis of super-active TALE (2015)
  • Author(s): Yoko Terahara, Kazuho Ikeda, Kenta Sumiyama, Naoyuki Miyashita, and Yasushi Okada
  • Organizer: ASCB annual meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Development and application of improved TALE protein (2015)
  • Author(s): 池田一穂、寺原陽子、隅山健太、岡田康志
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学(石川県金沢市)
  • Year and Date: 2015-09-13
• [Presentation] 高活性TALE蛋白質の開発とゲノム配列ライブイメージングへの応用 (2015)
  • Author(s): 池田一穂、寺原陽子、隅山健太、岡田康志
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール船堀(東京都江戸川区)
  • Year and Date: 2015-07-02
• [Presentation] 熱安定型高活性TALENの開発 (2015)
  • Author(s): 池田一穂、宮下尚之
  • Organizer: バイオスーパーコンピューティング研究会(BSCRC)「ウィンタースクール 2015」
  • Place of Presentation: 愛知県田原市中山町
  • Year and Date: 2015-01-30 – 2015-01-31
  • Invited
• [Presentation] Super-active TALEN with improved stability at 37 degree Celsius enables highly efficient and homogeneous gene knockout in mammalian embryos (2014)
  • Author(s): K. Ikeda, Y. Terahara, K. Sumiyama, N. Miyashita, Y. Sugita, Y. Okada
  • Organizer: The 2014 ASCB/IFCB meeting
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2014-12-06 – 2014-12-10
  • Invited