| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
One third of the proteins are synthesized in the endoplasmic reticulum (ER) and transported as cargo by transported vesicles (COPII vesicles) from the ER exit sites (ERES) to the Golgi apparatus. This mechanism is conserved in all eukaryotic cells. In this research, we aimed to elucidate the molecular mechanism of ERES formation.
By using the high-speed and high-resolution microscope SCLIM exceeding conventional fluorescent microscopy, we examined the localization of Sar1, which is a key regulator for COPII vesicle formation and has a function of membrane bending.
Sar1 showed some accumulation in ERES. High-resolution 3D observation indicated that Sar1 localized at the rims of the COPII vesicle membranes which were connected to the ER, but was excluded from the rest of the COPII vesicle membranes. Additionally, we showed that the reversible membrane association of Sar1 GTPase leads to its localization being restricted to the rims of COPII-coated membranes in vivo.
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