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The mechanism of functional sub-compartment formation in the endoplasmic reticulum by membrane structure

Research Project

Project/Area Number 26440110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kurokawa Kazuo  国立研究開発法人理化学研究所, 光量子工学研究領域, 専任研究員 (40333504)

Research Collaborator ISHII Midori  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords小胞体サブコンパートメント / ERES / COPII小胞 / Sar1 / 小胞体 / 3次元局在 / 輸送小胞 / ライブイメージング
Outline of Final Research Achievements

One third of the proteins are synthesized in the endoplasmic reticulum (ER) and transported as cargo by transported vesicles (COPII vesicles) from the ER exit sites (ERES) to the Golgi apparatus. This mechanism is conserved in all eukaryotic cells. In this research, we aimed to elucidate the molecular mechanism of ERES formation.
By using the high-speed and high-resolution microscope SCLIM exceeding conventional fluorescent microscopy, we examined the localization of Sar1, which is a key regulator for COPII vesicle formation and has a function of membrane bending.
Sar1 showed some accumulation in ERES. High-resolution 3D observation indicated that Sar1 localized at the rims of the COPII vesicle membranes which were connected to the ER, but was excluded from the rest of the COPII vesicle membranes. Additionally, we showed that the reversible membrane association of Sar1 GTPase leads to its localization being restricted to the rims of COPII-coated membranes in vivo.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (23 results)

All 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Remarks (4 results)

  • [Journal Article] Sar1 localizes at the rims of COPII-coated membranes in vivo.2016

    • Author(s)
      Kazuo Kurokawa, Yasuyuku Suda and Akihiko Nakano
    • Journal Title

      J. Cell Sci.

      Volume: 129 Issue: 17 Pages: 3231-3237

    • DOI

      10.1242/jcs.189423

    • NAID

      120007129359

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] COPI is essential for Golgi cisternal maturation and dynamics.2016

    • Author(s)
      Midori Ishii, Yasuyuki Suda, Kazuo Kurokawa, and Akihiko Nakano
    • Journal Title

      J. Cell Sci.

      Volume: 129 Issue: 17 Pages: 3251-3261

    • DOI

      10.1242/jcs.193367

    • NAID

      120007129358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ゴルジ体によるタンパク質輸送機構2016

    • Author(s)
      黒川量雄、中野明彦
    • Journal Title

      生物物理

      Volume: 56 Pages: 201-206

    • NAID

      130005165634

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Live-cell visualization of excitation energy dynamics in chloroplast thylakoid structures.2016

    • Author(s)
      Masakazu Iwai, Makio Yokono, Kazuo Kurokawa, Akira Ichihara, and Akihiko Nakano
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 29940-29940

    • DOI

      10.1038/srep29940

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER2014

    • Author(s)
      K. Kurokawa, M. Okamoto, and A. Nakano
    • Journal Title

      Nat. Commun

      Volume: 5 Issue: 1 Pages: 3653-3653

    • DOI

      10.1038/ncomms4653

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 超解像ライブイメージング-細胞内膜交通の新しいモデル2014

    • Author(s)
      黒川量雄
    • Journal Title

      現代化学

      Volume: 523号 Pages: 38-42

    • Related Report
      2014 Research-status Report
  • [Presentation] 4D imaging of membrane trafficking in living Saccharomyces cerevisiae.2016

    • Author(s)
      Kazuo Kurokawa
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      Awazi yumebutai International Conference Center (Awazi)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 積荷タンパク質のゴルジ体槽間輸送機構2016

    • Author(s)
      黒川量雄, 石井みどり, 中野明彦
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      シーサイドホテル舞子ビラ(神戸)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] 小胞体ゴルジ体間、ゴルジ体槽間のタンパク質輸送のイメージング2016

    • Author(s)
      黒川量雄
    • Organizer
      日本遺伝学会第88回大会
    • Place of Presentation
      日本大学国際関係学部 三島駅北口校舎(三島)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 積荷タンパク質のゴルジ体槽間輸送機構2016

    • Author(s)
      黒川量雄, 石井みどり, 中野明彦
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Sar1 concentrates at the neck of COPII-coated vesicle in vivo2015

    • Author(s)
      黒川量雄、須田恭之、中野明彦
    • Organizer
      BMB2015 (第38回日本分子生物学会年会/第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸ポートピアアイランド(兵庫)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Sar1 concentrates on the neck region of COPII-coated vesicle at the endoplasmic reticulum exit sites in vivo.2015

    • Author(s)
      黒川量雄、中野明彦
    • Organizer
      Cell biology of yeasts
    • Place of Presentation
      Cold spring Harbor laboratory (New York)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Studies on the molecular mechanism of cisternal maturation in budding yeast Sacharomyces cerevisiae2015

    • Author(s)
      石井みどり、黒川量雄、須田恭之、中野明彦
    • Organizer
      Cell biology of yeasts
    • Place of Presentation
      Cold spring Harbor laboratory (New York)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゴルジ体槽成熟におけるCOPIタンパク質の解析2015

    • Author(s)
      石井みどり、黒川量雄、須田恭之、中野明彦
    • Organizer
      酵母遺伝学フォーラム第48回研究報告会
    • Place of Presentation
      広島大学(広島)
    • Year and Date
      2015-09-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 細胞内膜交通の高速高感度高解像度イメージング2015

    • Author(s)
      黒川量雄、中野明彦
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船橋(東京)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Saccharomyces cerevisiaeのゴルジ体槽成熟におけるCOPIの機能解析2015

    • Author(s)
      石井みどり、黒川量雄、須田恭之、中野明彦
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船橋(東京)
    • Year and Date
      2015-06-30
    • Related Report
      2015 Research-status Report
  • [Presentation] Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER .2014

    • Author(s)
      Kazuo Kurokawa and Akihiko Nakano
    • Organizer
      the 2014 ASCB/IFCB meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2014-12-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 小胞体からゴルジ体へのタンパク質輸送機構の解明2014

    • Author(s)
      黒川量雄、中野明彦
    • Organizer
      酵母遺伝学フォーラム第47回研究報告会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2014-09-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 積荷タンパク質はcis-GolgiとERESの接触により小胞体からゴルジ体へ輸送される2014

    • Author(s)
      黒川量雄、中野明彦
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      東大寺総合文化センター(奈良)
    • Year and Date
      2014-06-11
    • Related Report
      2014 Research-status Report
  • [Remarks] Sar1の生細胞内三次元局在を可視化 -積荷タンパク質輸送小胞の鍵分子の限局を観察-

    • URL

      http://www.riken.jp/pr/press/2016/20160902_1/

    • Related Report
      2016 Annual Research Report
  • [Remarks] ゴルジ体槽成熟の分子機構を解明 -COPⅠ被覆タンパク質の機能が必須-

    • URL

      http://www.riken.jp/pr/press/2016/20160902_2/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 躍動する光合成反応を可視化 -超解像イメージングで生きた細胞の光エネルギー伝達を観察-

    • URL

      http://www.riken.jp/pr/press/2016/20160715_1/

    • Related Report
      2016 Annual Research Report
  • [Remarks] Protein transport by ‘hug-and-kiss’

    • URL

      http://www.riken.jp/en/research/rikenresearch/highlights/7802/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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