Research on the novel mechanism for cell cycle progresion through centriolar localization

• Project/Area Number: 26440111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: Inoko Akihito 愛知県がんセンター(研究所), 腫瘍医化学部, 主任研究員 (30393127)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞周期制御 / 中心体複製 / 中心小体 / 一次線毛 / 紡錘体 / 線毛根 / 第二次高調波 / 中心体 / 細胞周期

Outline of Final Research Achievements

Recently, we got a suggestion that our protein Albatross, that regulates cell-cell adhesion (Inoko et al. JCB2008), also contributes to all centriolar dynamics: centriole duplication, spindle formation and primary cilia formation. In this study, we clarified that the molecular basis of cell cycle progression by Albatross is its binding to (1) key players for centriole duplication and (2) key kinases for spindle formation. Taken together, Albatross is the first protein that integrates centrosomal microtubule dynamics. Thus, Albatross is the bi-player for both cell proliferation and cell differentiation.
Also, we succeeded in naive imaging of the Rootletin fiber which contributes to cell cycle regulation as a centrosome connection.

Research Products

• [Journal Article] SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation (2017)
  • Author(s): Toshihiro Akiyama, Akihito Inoko, Yuichi Kaji, Shigenobu Yonemura, Kisa Kakiguchi, Hiroki Segawa, Kei Ishitsuka, Masaki Yoshida, Osamu Numata, Philippe Leproux, Vincent Couderc, Tetsuro Oshika, Hideaki Kano
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 39967
  • DOI: 10.1038/srep39967
  • NAID: 120007135221
  • URL: https://tokushima-u.repo.nii.ac.jp/records/2005531
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. (2016)
  • Author(s): Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M.
  • Journal Title: J Cell Biol. Volume: 212 Issue: 4 Pages: 409-423
  • DOI: 10.1083/jcb.201507046
  • Peer Reviewed / Open Access
• [Journal Article] Cytokinetic failure-induced tetraploidy develops into aneuploidy, triggering skin aging in phospho-vimentin deficient mice. (2015)
  • Author(s): Tanaka, H., Goto, H., Inoko, A., Makihara, H., Enomoto, A., Horimoto, K., Matsuyama, M., Kurita, K., Izawa, I., and Inagaki, M.
  • Journal Title: J. Biol. Chem. Volume: 290 Issue: 21 Pages: 12984-12998
  • DOI: 10.1074/jbc.m114.633891
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 非標識イメージング技術のインパクトをあげる分子生物学の利活用 (2017)
  • Author(s): 猪子 誠人, 加納 英明
  • Organizer: 第69回日本細胞生物学会大会 シンポジウム口演
  • Place of Presentation: 仙台国際センター（仙台市）
• [Presentation] Albatross/Fbf1 蛋白質は中心体動態を統合する (2016)
  • Author(s): 猪子 誠人，林 裕子，清野 透，稲垣 昌樹
  • Organizer: 第68回日本細胞生物学会・第11回日本ケミカルバイオロジー学会 合同大会 ワークショップ口演
  • Place of Presentation: 京都テルサ（京都市）
• [Presentation] Albatross/Fbf1蛋白質は中心体機能に広く寄与する (2015)
  • Author(s): 猪子誠人、林裕子、清野透、稲垣昌樹
  • Organizer: 第38回分子生物学会年会 第88回 日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-02
• [Presentation] Centriolar Albatross contributes to duplication of centrioles (2014)
  • Author(s): Akihito Inoko, Yuko Hayashi, Masaki Inagaki
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2014-11-25
• [Presentation] Albatross蛋白質は中心小体の複製に寄与する (2014)
  • Author(s): Akihito Inoko, Yuko Hayashi, Masaki Inagaki
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館、京都市
  • Year and Date: 2014-10-18
• [Remarks] Researchmap 猪子誠人
  • URL: http://researchmap.jp/read0164677/
• [Remarks] つくばサイエンスニュース：視覚生み出す網膜の微細構造可視化―他の感覚器に応用も
  • URL: http://www.tsukuba-sci.com/?p=1406
• [Remarks] 網膜内構造の「そのまま」見える化に成功～新しい非線形光学顕微鏡の効果を実証～
  • URL: http://www.pref.aichi.jp/soshiki/byoin-kanri/290106soumu.html
• [Remarks] 筑波大ら，白色レーザーで標識物質を使わずイメージング
  • URL: http://www.optronics-media.com/news/20170110/45287/
• [Remarks] 網膜内構造の「そのまま」見える化に成功筑波大学 愛知県がんセンター研究所 研究グループ
  • URL: https://www.adcom-media.co.jp/news/2017/01/25/24906/
• [Remarks] Researchmap 猪子誠人
  • URL: https://researchmap.jp/read0164677/
• [Remarks] 愛知県がんセンター研究所 腫瘍医化学部
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/08hatsugan_seigyo/