Functional analysis of a novel PCP regulator

• Project/Area Number: 26440113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Akita University
• Principal Investigator: Ayukawa Tomonori 秋田大学, 医学系研究科, 助教 (80586165)
• Co-Investigator(Kenkyū-buntansha): 山崎 正和 秋田大学, 医学(系)研究科(研究院), 准教授 (40373378)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 平面内細胞極性 / PCP / 発生生物学 / 遺伝学

Outline of Final Research Achievements

Most tissues and organs acquire a polarity within the plane of the epithelium, which is orthogonal to the axis of apico-basal polarity. This is called planar cell polarity (PCP). PCP pathway is an evolutionarily conserved mechanism in multicellular organism.
PCP molecules are categorized into two groups by its function. In previous study we have already succeeded to find a novel group of PCP regulators (Jbug group). In this study we try to understand molecular mechanism by which Jbug group regulates the PCP pathway.

Research Products

• [Journal Article] Dual roles of O-glucose glycans redundant with monosaccharide O-fucose on Notch in Notch Trafficking. (2016)
  • Author(s): Matsumoto, K., Ayukawa, T., Ishio, A., Sasamura, T., Yamakawa, T. and Matsuno, K.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 26 Pages: 13743-13752
  • DOI: 10.1074/jbc.m115.710483
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dachsous 依存的な Spiny-legs の非対称局在はショウジョウバエに おける平面内細胞極性の方向を決定する (2015)
  • Author(s): 鮎川友紀
  • Journal Title: Akita J Med Volume: 42 Pages: 9-17
  • NAID: 120005624821
• [Journal Article] O-fucose monosaccharide of Drosophila Notch has a temperature-sensitive function and cooperates with O-glucose glycan in Notch transport and Notch signaling activation (2015)
  • Author(s): Akira Ishio, Takeshi Sasamura, Tomonori Ayukawa, Junpei Kuroda, Hiroyuki O. Ishikawa, Naoki Aoyama, Kenjiroo Matsumoto, Takuma Gushiken, Tetsuya Okajima, Tomoko Yamakawa and Kenji Matsuno2
  • Journal Title: Jounal of Biochemistry Volume: 290 Issue: 1 Pages: 505-519
  • DOI: 10.1074/jbc.m114.616847
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dachsous-Dependent Asymmetric Localization of Spiny-Legs Determines Planar Cell Polarity Orientation in Drosophila. (2014)
  • Author(s): Ayukawa T., Akiyama M., Mummery-Widmer JL., Stoeger T., Sasaki J., Knoblich JA., Senoo H., Sasaki T., Yamazaki M.
  • Journal Title: Cell Rep. Volume: 8 Issue: 2 Pages: 610-621
  • DOI: 10.1016/j.celrep.2014.06.009
  • Peer Reviewed / Open Access
• [Presentation] 平面内細胞極性を司る新規調節機構の解析 (2017)
  • Author(s): 鮎川友紀
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス（長崎）
  • Year and Date: 2017-03-28
• [Presentation] 平面内細胞極性を司る新規調節機構の解析 (2016)
  • Author(s): 鮎川友紀
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2016-11-30
• [Presentation] Dachsous依存的なSpiny-legsの非対称局在はショウジョウバエにおける平面内細胞極性の方向を決定する (2015)
  • Author(s): 鮎川友紀
  • Organizer: 日本解剖学会第61回東北・北海道連合支部学術集会
  • Place of Presentation: 盛岡観光文化交流センター プラザおでって（盛岡）
  • Year and Date: 2015-08-29
• [Presentation] Dachsous-dependent asymmetric localization of Spiny-legs is critical for determining planar cell polarityorientation in Drosophila (2014)
  • Author(s): Ayukawa T
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] A molecular mechanism underlying planar cell polarity orientation in Drosophila (2014)
  • Author(s): Ayukawa T
  • Organizer: International symposium on“Homeostasis through development, life, and diseases.”
  • Place of Presentation: 群馬大学 昭和キャンパス 刀城会館（高崎）
  • Year and Date: 2014-11-07
• [Presentation] Dachsous-dependent polarization of Spiny-legs is critical for determining planar cell polarity orientation in Drosophila (2014)
  • Author(s): Ayukawa T
  • Organizer: Japanese Drosophila Research Conference 11 (JDRC11)
  • Place of Presentation: 金沢歌劇座（金沢）
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Dachsous-dependent polarization of Spiny-legs is critical for determining planar cell polarity orientation in Drosophila (2014)
  • Author(s): Ayukawa T
  • Organizer: 47th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: ウインクあいち（名古屋）
  • Year and Date: 2014-05-27 – 2014-05-30