| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this project, we studied involvement of cell tension and cell shape for neural-epidermal patterning with Xenopus embryos. (1) We validated the FRET tension probes we made and showed they worked properly. Interestingly, cell tension was obviously different between neural and epidermal ectodermal. We also showed the change of cell tension by injecting with several factors as SoxD. (2) Inhibition of vinculin and actinin altered the localization of actin fiber in ectodermal cells. (3) We performed the profiling of the ectoderm cell shape. By these analyses, we found that the apical area and the orientation of cells showed difference between epidermal and neuroectoderm cells. These data suggest that both types of cells give rise to distinct strength depending on cell fate, resulting in the change of cell shape and tension and contributing determination of ectodermal pattern.
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