Temporal control of molecular function in axon wiring of the visual system
| Project/Area Number |
26440119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 軸索投射 / 遺伝子発現 / 視神経 / ショウジョウバエ / 時間的制御 |
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| Outline of Final Research Achievements |
To know the temporal requirement of the molecules involved in axon wiring of Drosophila visual system, we genetically manipulated the gene expression during pupal stage and observed the projection pattern of the axons in the adult. We found that the receptor tyrosine phosphatases LAR and Ptp69D play a critical role from 25% to 50% after puparium formation (APF). We also tested the temporal effect of overexpression of two transmembrane proteins, Golden goal and Flamingo, which is known to cause the R7 photoreceptor axons to terminate at the wrong layer. The effect was observed when the protein was expressed at 24hours APF but not later. These results indicate that the protein function is required only at very limited range of time during pupal stage for proper wiring of visual system axons.
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Report
(4 results)
Research Products
(11 results)
