Mechanisms of male-type differentiation in mouse primordial germ cells

• Project/Area Number: 26440120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Yokohama National University
• Principal Investigator: Suzuki Atsushi 横浜国立大学, 大学院工学研究院, 准教授 (60467058)
• Research Collaborator: NIIMI Yuki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖細胞 / Nanos / Dead end / 精巣テラトーマ / 潜在的な分化多能性 / 精巣性テラトーマ / 始原生殖細胞 / テラトーマ / RNA結合タンパク質

Outline of Final Research Achievements

Mouse NANOS2 suppresses ectopic meiosis and promotes male-type gene expression, playing a critical role in sexual differentiation of primordial germ cells. However, the mechanisms involved in target specificity remain elusive. We show that another RBP, Dead end1 (DND1), directly interacts with NANOS2 to load unique RNAs into the CNOT complex. Thus, DND1 is an essential partner for NANOS2 that leads to the degradation of specific RNAs.

Research Products

• [Journal Article] Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development. (2016)
  • Author(s): Suzuki A, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y.
  • Journal Title: EMBO Rep. Volume: 17(1) Issue: 1 Pages: 37-46
  • DOI: 10.15252/embr.201540828
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells. (2014)
  • Author(s): Suzuki A, Niimi Y, Saga Y.
  • Journal Title: Biol Open. Volume: 3 Issue: 12 Pages: 1207-16
  • DOI: 10.1242/bio.20149308
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Functional analysis of mouse Dead end1 in the maintenance of spermatogonial cells (2016)
  • Author(s): Niimi Y., Kikuchi A., Tazu Y., Suzuki A.
  • Organizer: The 2016 meetings on GERM CELLS
  • Place of Presentation: Cold Spring Harbor Laboratory, New York
  • Year and Date: 2016-10-04
  • Int'l Joint Research
• [Presentation] Mouse Dead end 1 is required for the maintenance of spermatogonial cells (2015)
  • Author(s): 菊池藍、新見夕姫、鈴木敦
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Functional analysis of mouse DAZL in spermatogenesis (2015)
  • Author(s): Yuki Niimi , Atsushi Suzuki
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] DND1 is an essential partner of NANOS2 for selective binding to unique RNAs. (2014)
  • Author(s): Atsushi Suzuki
  • Organizer: Germ Cell meeting, Cold Spring Harbor Laboratory
  • Place of Presentation: Cold Spring Harbor Laboratory, New York, USA
  • Year and Date: 2014-10-07 – 2014-10-11
• [Presentation] マウス生殖細胞雄性分化におけるDead end1の機能解析 (2014)
  • Author(s): 新見夕姫
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 長浜バイオ大学, 滋賀県, 日本
  • Year and Date: 2014-09-17 – 2014-09-19