Analysis of molecular mechanisms controlling fate choice of pigment cells

• Project/Area Number: 26440121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: Hashimoto Hisashi 名古屋大学, 生物機能開発利用研究センター, 助教 (30359757)
• Co-Investigator(Renkei-kenkyūsha): NARUSE Kiyoshi 基礎生物学研究所, 進化多様性生物学領域, 特任教授 (50208089)
• Research Collaborator: NAGAO Yusuke ; TAKADA Hiroyuki ; MYADAI Motohiro
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 転写因子 / 分化制御 / 転写制御因子 / 進化 / 細胞分化 / 多様性 / 運命決定 / 細胞運命決定

Outline of Final Research Achievements

How individual cell fates become specified from multipotent progenitors is a fundamental question in developmental biology. Pigment cells derive from a multipotent progenitor, but while in zebrafish there are three types of pigment cells (melanocytes, iridophores and xanthophores), in medaka the progenitors form four (as zebrafish, plus leucophores), raising questions about how conserved mechanisms of fate specification of each pigment cell type are in these fish. To address them, we focus on Sox5 and Sox10, which we previously showed were involved in pigment cell development in medaka and zebrafish, respectively. We show that development of all pigment cells, except leucophores, is dependent on Sox10, and that Sox5 modulates Sox10 activity antagonistically in all pigment cells in zebrafish, and melanocytes and iridophores in medaka. In medaka, Sox5 acts cooperatively with Sox10 to promote xanthophore fate and to repress leucophore fate.

Research Products

• [Int'l Joint Research] バース大学(英国)
• [Int'l Joint Research] University of Bath(United Kingdom)
• [Journal Article] Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. (2018)
  • Author(s): Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H
  • Journal Title: PLoS Genetics Volume: 14(4) Issue: 4 Pages: 1007260-1007260
  • DOI: 10.1371/journal.pgen.1007260
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering (2018)
  • Author(s): Watakabe Ikuko、Hashimoto Hisashi、Kimura Yukiko、Yokoi Saori、Naruse Kiyoshi、Higashijima Shin-ichi
  • Journal Title: Zoological Letters Volume: 4 Issue: 1 Pages: 3-3
  • DOI: 10.1186/s40851-017-0086-3
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of medaka orthologue of human transglutaminase 2 (OlTGT) (2017)
  • Author(s): Takada Y., Watanabe Y., Okuya K., Tatsukawa H., Hashimoto H, Hitomi K.
  • Journal Title: Biochem. Biotechnol. Biosci. Volume: 81 Issue: 3 Pages: 469-474
  • DOI: 10.1080/09168451.2016.1256757
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Osteocrin, a secretory peptide from heart, contributes to cranial osteogenesis in zebrafish (2016)
  • Author(s): Chiba A, Watanabe-Takano H, Terai K, Fukui H, Miyazaki T, Uemura M, Hashimoto H, Hibi M, Fukuhara S, Mochizuki N
  • Journal Title: Development Volume: 144 Pages: 334-344
  • DOI: 10.1242/dev.143354
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Establishment of oct4:egfp transgenic and oct4:egfp /b-actin:DsRed double transgenic medaka lines (2016)
  • Author(s): Yokota S, Matsuno R, Kato H, Hashimoto H, Kinoshita M, Yokoi H, Suzuki T
  • Journal Title: In Vitro Cellular & Developmental Biology - Animal Volume: - Issue: 6 Pages: 646-653
  • DOI: 10.1007/s11626-016-0020-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] What is a vertebrate pigment cell? (2015)
  • Author(s): Schartl M, Larue L, Goda M, Bosenberg MW, Hashimoto H, Kelsh RN.
  • Journal Title: Pigment Cell and Melanoma Research Volume: 29 Issue: 1 Pages: 8-14
  • DOI: 10.1111/pcmr.12409
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Taking striping up a notch. (2014)
  • Author(s): Goda M, Kelsh RN, Hashimoto H
  • Journal Title: Pigment Cell and Melanoma Research Volume: 27 Issue: 5 Pages: 688-689
  • DOI: 10.1111/pcmr.12288
  • Peer Reviewed / Open Access
• [Journal Article] Leucophores are similar to xanthophores in their specification and differentiation processes in medaka (2014)
  • Author(s): T. Kimura, Y. Nagao, H. Hashimoto, Y. Yamamoto-Shiraishi, S. Yamamoto, T. Yabe, S. Takada, M. Kinoshita, A. Kuroiwa and K. Naruse
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Issue: 20 Pages: 1986767-1986767
  • DOI: 10.1073/pnas.1311254111
  • Peer Reviewed / Open Access
• [Journal Article] Sox5 functions as a fate switch in medaka pigment cell development. (2014)
  • Author(s): Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H
  • Journal Title: PLoS genetics Volume: 10(4) Issue: 4 Pages: e1004246-e1004246
  • DOI: 10.1371/journal.pgen.1004246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multiple pigment cell types contribute to the black, blue, and orange ornaments of male guppies (Poecilia reticulata). (2014)
  • Author(s): Kottler VA, Koch I, Floetenmeyer M, Hashimoto H, Weigel D, Dreyer C
  • Journal Title: PLoS ONE Volume: 9(1) Issue: 1 Pages: e85647-e85647
  • DOI: 10.1371/journal.pone.0085647
  • Peer Reviewed / Open Access
• [Presentation] Sox5 modulates Sox10-mediated pigment cell fate specification in medaka and zebrafish. (2017)
  • Author(s): Nagao Y, Takada H, Miyadai M, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H.
  • Organizer: Neural Crest and Cranial Placodes, Gordon Research Conference
  • Place of Presentation: Ventura CA, USA
  • Year and Date: 2017-02-05
  • Int'l Joint Research
• [Presentation] A variegated medaka mutant Va: a model of human Carney complex. (2016)
  • Author(s): Goda M, Shizuka T, Inoue C, Nishikawa K, Miki C, Kimura T, Villareal MO, Isoda H, Naruse K, Hibi M, Wakamatsu Y, Hashimoto H.
  • Organizer: 第８７回日本動物学会年会
  • Place of Presentation: Milan, Italy
  • Year and Date: 2016-11-14
• [Presentation] Sox5 modulates Sox10-mediated pigment cell fate specification in medaka and zebrafish. (2016)
  • Author(s): Nagao Y, Takada H, Miyadai M, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H.
  • Organizer: European Society for Pigment Cell Research
  • Place of Presentation: Milan, Italy
  • Year and Date: 2016-09-12
  • Int'l Joint Research
• [Presentation] A variegated medaka mutant Va: a model of human Carney complex. (2016)
  • Author(s): Goda M, Shizuka T, Inoue C, Nishikawa K, Miki C, Kimura T, Villareal MO, Isoda H, Naruse K, Hibi M, Wakamatsu Y, Hashimoto H.
  • Organizer: European Society for Pigment Cell Research
  • Place of Presentation: Milan, Italy
  • Year and Date: 2016-09-12
  • Int'l Joint Research
• [Presentation] Sox5 modulates Sox10-meiated pigment cell fate specification in medaka and zebrafish. (2016)
  • Author(s): Nagao Y, Takada H, Miyadai M, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H.
  • Organizer: 第２２回小型魚類研究会
  • Place of Presentation: 岡崎
  • Year and Date: 2016-08-20
• [Presentation] Generation of zebrafish sox5 mutant by CRISPR for understanding the mechanism of fate specification of pigment cells in teleost. (2015)
  • Author(s): Takada H, Nagao Y, Adachi T, Hibi M, Kelsh RN, Hashimoto H
  • Organizer: 第２１回小型魚類研究会
  • Place of Presentation: 大阪
  • Year and Date: 2015-09-19
• [Presentation] Roles of sox10 and its possible interaction with sox5 in pigment cell development. (2015)
  • Author(s): Nagao Y, Takada H, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H
  • Organizer: European Society for Pigment Cell Research
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2015-09-15
  • Int'l Joint Research
• [Presentation] Generation of zebrafish sox5 mutant by CRISPR for understanding the mechanism of fate specification of pigment cells in teleost. (2015)
  • Author(s): Takada H, Nagao Y, Adachi T, Hibi M, Kelsh RN, Hashimoto H
  • Organizer: 7th European Zebrafish Meeting
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] Roles of Sox10 and its possible interaction with Sox5 in pigment cell development. (2015)
  • Author(s): Nagao Y, Takada H, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H
  • Organizer: 7th European Zebrafish Meeting
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] Roles of sox10 and its possible interaction with sox5 in pigment cell development of medaka. (2015)
  • Author(s): Nagao Y, Takada H, Seki R, Kamei Y, Hara I, Kimura T, Taniguchi Y, Naruse K, Kelsh RN, Hibi M, Hashimoto H
  • Organizer: 第４８回日本発生生物学会年会
  • Place of Presentation: 筑波
  • Year and Date: 2015-06-02
• [Presentation] Roles of sox10 and its genetic interaction with sox5 in pigment cell development. (2014)
  • Author(s): Nagao Y, Takada H, Seki R, Kamei Y, Hara I, Kimura T, Taniguchi Y, Naruse K, Kelsh RN, Hibi M, Hashimoto H
  • Organizer: 第２０回小型魚類研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-20 – 2014-09-21
• [Presentation] Sox5 controls a fate choice during pigment cell development in medaka. (2014)
  • Author(s): Nagao Y, Adachi T, Shimizu A, Seki R, Inoue C, Kamei Y, Hara I, Kimura T, Taniguchi Y, Naruse K, Kelsh RN, Wakamatsu Y, Hibi M, Hashimoto H
  • Organizer: 第４7回日本発生生物学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Sox5 functions as a fate switch in medaka pigment cell development. (2014)
  • Author(s): Hashimoto H
  • Organizer: 2nd Strategical Meeting for Medaka Research
  • Place of Presentation: セビリア、スペイン
  • Year and Date: 2014-04-10 – 2014-04-12