| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Hes1 regulates the maintenance and the differentiation of stem cells by its dynamic expression. Previous studies demonstrated that Hes1 expression oscillates in stem cells, and that the oscillation contributes to their potency and differentiation fates. However, the molecular mechanisms regulating the oscillatory expression of Hes1 protein have not been fully identified.
We have focused on the post-translational regulation of Hes1 protein and tried to identify regulatory factors from the binding partners of Hes1 protein. We identified Usp27x and its homologs as new deubiquitinases (DUBs) of Hes1. We reported that these DUBs stabilize and modulate Hes1 protein dynamics by removing ubiquitin molecules, and thereby regulate neuronal differentiation of stem cells (Kobayashi et al, 2015). Moreover, we identified some amino acid residues crucial for Hes1 functions by post-translational regulation. We also identified new factors bound to recombinant Hes1.
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