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Molecular analysis of the stem cell nature in the amphibian model of retinal regeneration

Research Project

Project/Area Number 26440125
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionNara Medical University (2015-2017)
Nara Women's University (2014)

Principal Investigator

Araki Masasuke  奈良県立医科大学, 医学部, 研究員 (00118449)

Co-Investigator(Kenkyū-buntansha) 小林 千余子  奈良県立医科大学, 医学部, 講師 (20342785)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords網膜再生 / 網膜色素上皮細胞 / 虹彩 / Xenopsu / ブタ / 分化転換 / 網膜色素上皮 / Xenopus / chick / 組織培養 / 炎症 / Matrix metalloproteinase / 幹細胞 / 毛様体 / N-cadherin / Gap junction
Outline of Final Research Achievements

Amphibians are able to regenerate the whole retinal tissue when surgically removed. This ability had been considered to be limited only to the urodeles until Araki's group discovered that the complete regeneration occurs also in Xenopus. We established different organ-culture systems in which the retinal pigment epithelial (RPE) cells transdifferentiate into retinal cells. In the present study, we analyzed the interaction of RPE cells and basement membrane and the initiation of inflammatory response. The results indicate that inflammatory cytokines induce Matrix metalloproteinase (MMP) gene expression, which subsequently induces RPE cells to detach from the basement membrane. We also attempted to reveal the stem cell nature of the porcine iris tissue-derived cells by using the Matrigel embedding culture, and found that immediately after transferred to the culture, these cells differentiate into neural cells. This will favor the future analysis of stem cell nature of porcine RPE cells.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 5 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] Univeristy of Leicester(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Darmstadt University of Technology(Germany)

    • Related Report
      2015 Research-status Report
  • [Journal Article] A novel culture method reveals unique neural stem/progenitors in mature porcine iris tissues that differentiate into neuronal and rod photoreceptor-like cells2017

    • Author(s)
      Royall Lars N.、Lea Daniel、Matsushita Tamami、Takeda Taka-Aki、Taketani Shigeru、Araki Masasuke
    • Journal Title

      Brain Research

      Volume: 1675 Pages: 51-60

    • DOI

      10.1016/j.brainres.2017.08.027

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Upregulation of matrix metalloproteinase triggers transdifferentiation of retinal pigmented epithelial cells in Xenopus laevis: A Link between inflammatory response and regeneration2017

    • Author(s)
      Naitoh Hanako、Suganuma Yukari、Ueda Yoko、Sato Takahiko、Hiramuki Yosuke、Fujisawa-Sehara Atsuko、Taketani Shigeru、Araki Masasuke
    • Journal Title

      Developmental Neurobiology

      Volume: 77 Issue: 9 Pages: 1086-1100

    • DOI

      10.1002/dneu.22497

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Upregulation of Matrix Metalloproteinase Triggers Transdifferentiation of Retinal Pigmented Epithelial Cells in Xenopus laevis: A Link Between Inflammatory Response and Regeneration2017

    • Author(s)
      Hanako Naitoh, Yukari Suganuma, Yoko Ueda, Takahiko Sato, Yosuke Hiramuki, Atsuko Fujisawa-Sehara, Shigeru Taketani, Masasuke Araki
    • Journal Title

      Developmental Neurobiology

      Volume: -

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Localization of Neurensin1 in cerebellar Purkinje cells of the developing chick and its possible function in dendrite formation.2016

    • Author(s)
      Kiyonaga-Endou K, Oshima M, Sugimoto K, Thomas M, Taketani S and Araki M
    • Journal Title

      Brain Research

      Volume: 1635 Pages: 113-120

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A new Xenopus model discloses a novel mode of retinal regeneration2015

    • Author(s)
      Masasuke Araki
    • Journal Title

      Neural Reg. Res.

      Volume: 9 Pages: 2125-2127

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Retinal stem/progenitor cells in the ciliary marginal zone complete the whole retina regeneration: A study of retinal regeneration in a novel animal model.2014

    • Author(s)
      Ayumi Miyake and Masasuke Araki
    • Journal Title

      Developmental Neurobiology

      Volume: 74 Pages: 739-756

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Abnormal retinal development associated with FRMD7 mutations.2014

    • Author(s)
      Thomas MG, Crosier M, S Lindsay, A Kumar, M Araki, B P Leroy, R J McLean, V Sheth, G Maconachie, S Thomas, A T Moore, I Gottlob
    • Journal Title

      Human Mol. Genetics

      Volume: 23 Pages: 4086-4093

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immediate differentiation of neuronal cells from stem/progenitor-like cells of the avian iris tissues.2014

    • Author(s)
      Matsushita T, Fujihara A, Royall L, Kagiwada S, Kosaka M and Araki M
    • Journal Title

      Exp. Eye Res.

      Volume: 123 Pages: 16-26

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Xenopus momdel of retinal regeneration as an approach to the development of Mmmalian ocular tissue stem cells2016

    • Author(s)
      Araki M
    • Organizer
      International Meeting on Aquatic Model Organisms for human diseases and Toxicology Research
    • Place of Presentation
      Okazaki, Aichi
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Regeneration of the retina and tissue engineering of neural stem cells using a three-dimensional culture2015

    • Author(s)
      Araki M
    • Organizer
      2015 Tissue Engineering Congress
    • Place of Presentation
      London, UK
    • Year and Date
      2015-09-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 眼組織に存在する網膜幹細胞の研究2015

    • Author(s)
      荒木正介
    • Organizer
      第4回 四大学合同発生/再生研究会
    • Place of Presentation
      鳥取県、米子市
    • Year and Date
      2015-08-31
    • Related Report
      2015 Research-status Report
  • [Presentation] アフリカツメガエル網膜再生モデルの有用性:これまでの成果を哺乳類網膜再生へいかにつなぐか?2015

    • Author(s)
      荒木正介
    • Organizer
      第1回次世代両生類研究会
    • Place of Presentation
      愛知県、岡崎市
    • Year and Date
      2015-08-24
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] Xenopus Development2014

    • Author(s)
      Masasuke Araki (分担執筆)
    • Total Pages
      411
    • Publisher
      John Wiley & Sons. Inc.
    • Related Report
      2014 Research-status Report
  • [Remarks] 奈良医科大学生物学教室研究紹介(荒木正介)

    • URL

      http://www.naramed-u.ac.jp/~biol/site/araki.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 奈良女子大学 神経発生研究室の研究紹介

    • URL

      http://www.nara-wu.ac.jp/bio/develop/menu.htm

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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