Molecular analysis of the stem cell nature in the amphibian model of retinal regeneration
| Project/Area Number |
26440125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Nara Medical University (2015-2017)
Nara Women's University (2014) |
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Principal Investigator |
Araki Masasuke 奈良県立医科大学, 医学部, 研究員 (00118449)
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| Co-Investigator(Kenkyū-buntansha) |
小林 千余子 奈良県立医科大学, 医学部, 講師 (20342785)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 網膜再生 / 網膜色素上皮細胞 / 虹彩 / Xenopsu / ブタ / 分化転換 / 網膜色素上皮 / Xenopus / chick / 組織培養 / 炎症 / Matrix metalloproteinase / 幹細胞 / 毛様体 / N-cadherin / Gap junction |
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| Outline of Final Research Achievements |
Amphibians are able to regenerate the whole retinal tissue when surgically removed. This ability had been considered to be limited only to the urodeles until Araki's group discovered that the complete regeneration occurs also in Xenopus. We established different organ-culture systems in which the retinal pigment epithelial (RPE) cells transdifferentiate into retinal cells. In the present study, we analyzed the interaction of RPE cells and basement membrane and the initiation of inflammatory response. The results indicate that inflammatory cytokines induce Matrix metalloproteinase (MMP) gene expression, which subsequently induces RPE cells to detach from the basement membrane. We also attempted to reveal the stem cell nature of the porcine iris tissue-derived cells by using the Matrigel embedding culture, and found that immediately after transferred to the culture, these cells differentiate into neural cells. This will favor the future analysis of stem cell nature of porcine RPE cells.
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] Abnormal retinal development associated with FRMD7 mutations.2014
Author(s)
Thomas MG, Crosier M, S Lindsay, A Kumar, M Araki, B P Leroy, R J McLean, V Sheth, G Maconachie, S Thomas, A T Moore, I Gottlob
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Journal Title
Human Mol. Genetics
Volume: 23
Pages: 4086-4093
Related Report
Peer Reviewed / Open Access
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