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Comprehensive analysis to identify the key molecules for sexual maturation, mating-type expression and the initiation of sexual reproduction in ciliate.

Research Project

Project/Area Number 26440126
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionNara Women's University

Principal Investigator

Sugiura Mayumi  奈良女子大学, 自然科学系, 准教授 (60397841)

Co-Investigator(Renkei-kenkyūsha) Harumoto Terue  奈良女子大学, 自然科学系, 教授 (80198936)
Research Collaborator Yuasa Hajime J  高知大学, 理工学部, 准教授 (40322797)
Project Period (FY) 2014-02-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords繊毛虫 / 接合 / 接合型 / 性成熟 / 交配フェロモン
Outline of Final Research Achievements

The main aim of the present study was to conduct a comprehensive analysis to identify the key molecules involved in the mechanisms of sexual maturation, mating-type expression and the initiation of sexual reproduction (conjugation) in ciliate. We have used the putative ancestral species of ciliate, Blepharisma stoltei, in which conjugation is induced between complementary mating type cells, I and II, under food-deprived conditions. First, we produced progeny strains by crossing type I and II cells, and observed precisely their mating-type expression patterns during sexual maturation. Then, we performed de novo RNA-seq analysis to compare gene expression among sexually immature cells and mature-type I and II cells. Finally, from the constructed transcriptome data, we have identified the enzyme involved in the biosynthetic pathway of mating pheromone, gamone 2, which is a tryptophan-derivative compound secreted by type II cells.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (23 results)

All 2018 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (19 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] A single amino acid residue regulates the substrate affinity and specificity of indoleamine 2,3-dioxygenase.2018

    • Author(s)
      Yuasa Hajime J.、Sugiura Mayumi、Harumoto Terue
    • Journal Title

      Archives of Biochemistry and Biophysics

      Volume: 640 Pages: 1-9

    • DOI

      10.1016/j.abb.2017.12.019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel Specificity of IDO Enzyme Involved in the Biosynthesis of Mating Pheromone in the Ciliate Blepharisma stoltei2017

    • Author(s)
      Sugiura Mayumi、Yuasa Hajime J.、Harumoto Terue
    • Journal Title

      Protist

      Volume: 168 Issue: 6 Pages: 686-696

    • DOI

      10.1016/j.protis.2017.09.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid response to nutrient depletion on the expression of mating pheromone, gamone 1, in Blepharisma japonicum.2016

    • Author(s)
      Sugiura M, Yamanaka M, Suzaki T and Harumoto T
    • Journal Title

      Jpn. J. Protozool.

      Volume: 49

    • NAID

      130005249727

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Two possible barriers blocking conjugation between different megakaryotypes of Blepharisma2015

    • Author(s)
      Mayumi Kobayashi, Mamiko Miura, Mari Takusagawa, Mayumi Sugiura, and Terue Harumoto
    • Journal Title

      Zoological Science

      Volume: 32 (1) Issue: 1 Pages: 53-61

    • DOI

      10.2108/zs140151

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ブレファリズマの環境応答「共食い・接合・休眠」2017

    • Author(s)
      杉浦真由美
    • Organizer
      日本動物学会第88回大会、シンポジウム「原生生物の魅力と研究材料としての有用性」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Giant Formation and Surviving Strategy in Blepharisma2017

    • Author(s)
      Harumoto T, Ono Y, Sugiura M
    • Organizer
      XV International Congress of Protistology-ICOP
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 繊毛虫Blepharisma属における異種間接合を防ぐ仕組みの解明に向けて2017

    • Author(s)
      杉野亜優、杉浦真由美、春本晃江
    • Organizer
      第50回日本原生生物学会大会・第1回日本共生生物学会大会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トリプトファン分解酵素の分子進化の新局面 IX2017

    • Author(s)
      湯浅創、杉浦真由美、春本晃江
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 繊毛虫ブレファリズマにおいてガモン2生合成に関与する新規IDOの同定2016

    • Author(s)
      杉浦真由美・湯浅創・春本晃江
    • Organizer
      日本原生生物学会第49回大会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] 繊毛虫Blepharismaの異なるmegakaryotype間での異種間接合2016

    • Author(s)
      杉野亜優・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第49回大会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Blepharisma hyalinumの表層顆粒の機能の探索2016

    • Author(s)
      田村望・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第49回大会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] ブレファリズマの性成熟過程における接合型発現パターンと遺伝子発現解析2015

    • Author(s)
      杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第48回大会
    • Place of Presentation
      国立感染症研究所戸山庁舎(東京都)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
  • [Presentation] Blepharisma hyalinum の分類の再検討2015

    • Author(s)
      小林真弓・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第48回大会
    • Place of Presentation
      国立感染症研究所戸山庁舎(東京都)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
  • [Presentation] ブレファリズマのジャイアント形成の意義と形成条件の検討2015

    • Author(s)
      尾野優奈・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第48回大会
    • Place of Presentation
      国立感染症研究所戸山庁舎(東京都)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 繊毛虫ブレファリズマにおける交配フェロモンの多様性と種分化2015

    • Author(s)
      春本晃江・小林真弓・杉浦真由美・篠原きよの・田草川真里
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Mating type expression during sexual maturation in Blepharisma stoltei2015

    • Author(s)
      Sugiura M and Harumoto T
    • Organizer
      Ciliate Molecular Biology Conference
    • Place of Presentation
      Camerino, Italy
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mating pheromone gamone 1 plays an important role in speciation of the ciliate Blepharisma2015

    • Author(s)
      Kobayashi M, Nishihara Y, Miura M, Takusagawa M, Sugiura M and Harumoto T
    • Organizer
      Ciliate Molecular Biology Conference
    • Place of Presentation
      Camerino, Italy
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of induction of sexual reproduction in the ciliates2014

    • Author(s)
      杉浦真由美
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ブレファリズマの交配フェロモン(ガモン1)の糖鎖構造とその役割2014

    • Author(s)
      春本晃江・山岸由和・岩﨑祥子・杉浦真由美・小林真弓・飯尾英夫
    • Organizer
      日本原生生物学会第47回大会
    • Place of Presentation
      宮城教育大学(仙台市)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 繊毛虫Blepharisma属における交配フェロモンgamone1の多様性と種分化2014

    • Author(s)
      小林真弓・田草川真理・杉浦真由美・春本晃江
    • Organizer
      日本原生生物学会第47回大会
    • Place of Presentation
      宮城教育大学(仙台市)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 繊毛虫 Blepharisma における交配フェロモンの生合成にIDO は関与するか2014

    • Author(s)
      亀岡里江子・篠原きよの・杉浦真由美・春本晃江
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 繊毛虫ブレファリズマの分類の再検討2014

    • Author(s)
      小林真弓・杉浦真由美・春本晃江
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] ブレファリズマにおけるジャイアント形成要因の検討と口部構造の観察2014

    • Author(s)
      尾野優奈・杉浦真由美・春本晃江
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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