| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Chloroplast ribosomes include 59 proteins in equimolar ratios. Though their sizes vary significantly, these proteins should be synthesized as equal moles. The translation elongation rates of a large S2 mRNA and a small S16 mRNA coding regions are found to be similar. However, the translation initiation rate of S16 mRNA 5'-UTR was very low. This is due to the presence of an S16-specific translation repressor in a chloroplast soluble fraction. We propose a model that translation of ribosome protein synthesis is regulated by mRNA-specific factors.
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