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Comprehensive study of the unique photosynthetic system driven by far-red light

Research Project

Project/Area Number 26440141
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

Tsuchiya Tohru  京都大学, 地球環境学堂, 准教授 (20362569)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsシアノバクテリア / クロロフィルd / 光合成 / 遠赤色光 / トランスポゾンタギング
Outline of Final Research Achievements

Acaryochloris marina can utilize far-red light for photosynthesis, whereas most other oxygenic photosynthetic organisms cannot use the light. In this project, we aimed to develop the molecular genetic techniques for A. marina in order to elucidate the molecular mechanism of its unique photosynthetic system driven by far-red light. In the result of this study, we succeeded in producing transposon-tagged mutants of A. marina by transposon tagging. In addition, we also succeeded in developing vectors for CRISPR interference, new technique for the repression of gene expression.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2017 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (3 results)

  • [Journal Article] Development of a high-frequency in vivo transposon mutagenesis system for Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 79422014

    • Author(s)
      Watabe, K., Mimuro, M., Tsuchiya, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 1-2 Pages: 2017-2026

    • DOI

      10.1007/s11120-015-0082-4

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a high-frequency in vivo transposon mutagenesis system for Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 79422014

    • Author(s)
      Watabe, K., Mimuro, M., Tsuchiya, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 11 Pages: 2017-2026

    • DOI

      10.1093/pcp/pcu128

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] CRISPR干渉法を適用したシアノバクテリアの光合成色素組成の改変2017

    • Author(s)
      早川 諒、土屋 徹
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 広宿主域プラスミド由来のオールインワンCRISPRiベクターの開発2017

    • Author(s)
      土屋 徹
    • Organizer
      平成29年度ラン藻ゲノム交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular genetic analysis of the chlorophyll d-dominated cyanobacterium Acaryochloris marina2014

    • Author(s)
      Tsuchiya, T., Watabe, K.
    • Organizer
      International meeting "Photosynthesis Research for Sustainability - 2014"
    • Place of Presentation
      Pushchino (Russia)
    • Year and Date
      2014-06-02 – 2014-06-07
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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