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How plant villin regulates chloroplast anchoring

Research Project

Project/Area Number 26440143
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionOsaka University

Principal Investigator

Takagi Shingo  大阪大学, 理学研究科, 教授 (10192626)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords葉緑体アンカー / ビリン / アクチン細胞骨格
Outline of Final Research Achievements

In photosynthesizing plant cells, chloroplasts maintain their specific intracellular distribution patterns under specific environmental conditions, which contributes to the operation of optimal photosynthesis under fluctuating environment. Since the actin cytoskeleton has been known to play some roles in the maintenance of chloroplast distribution patterns, we ascertained a possible involvement of a Ca2+-sensitive actin-binding protein villin (VLN). One of the five villin molecular species of the model plant Arabidopsis thaliana, AtVLN2 is localized on chloroplasts. AtVLN2 functions in the actin reorganization around the chloroplasts induced by a change in Ca2+ concentration, so may be involved in the regulation of chloroplast anchoring in the cortical cytoplasm of leaf palisade cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (32 results)

All 2018 2017 2016 2015 2014 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (24 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Circumnutation and distribution of phytohormones in Vigna angularis epicotyls2017

    • Author(s)
      Iida Motoyuki、Takano Toshihiko、Matsuura Takakazu、Mori Izumi C.、Takagi Shingo
    • Journal Title

      Journal of Plant Research

      Volume: 131 Issue: 1 Pages: 165-178

    • DOI

      10.1007/s10265-017-0972-y

    • NAID

      40021448101

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of actin cytoskeleton for regulation of chloroplast anchoring2017

    • Author(s)
      Sakai Yuuki、Takagi Shingo
    • Journal Title

      Plant Signaling & Behavior

      Volume: 12 Issue: 10 Pages: e1370163-e1370163

    • DOI

      10.1080/15592324.2017.1370163

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant nuclei move to escape ultraviolet-induced DNA damage and cell death.2016

    • Author(s)
      Iwabuchi K., Hidema J., Tamura K., Takagi S., Hara-Nishimura I.
    • Journal Title

      Plant Physiology

      Volume: 170 Issue: 2 Pages: 678-685

    • DOI

      10.1104/pp.15.01400

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro phosphorylation assay of putative blue-light receptor phototropins using microsomal and plasma-membrane fractions prepared from Vallisneria leaves.2015

    • Author(s)
      Sakai Y., Inoue S., Takagi S.
    • Journal Title

      Bio-protocol

      Volume: 5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Observation of chloroplast movement in Vallisneria.2015

    • Author(s)
      Sakai Y., Takagi S.
    • Journal Title

      Bio-protocol

      Volume: 5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells.2015

    • Author(s)
      Sakai Y, Inoue S, Harada A, Shimazaki K and Takagi S
    • Journal Title

      J. Integ. Plant Biol.

      Volume: 57 Issue: 1 Pages: 93-105

    • DOI

      10.1111/jipb.12284

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nuclear lamina localized at the nuclear periphery in interphase and at chromosomes in mitotic phase.2014

    • Author(s)
      Sakamoto Y., Takagi S.
    • Journal Title

      Atlas of Plant Cell Structure

      Volume: 26 Pages: 6-7

    • Related Report
      2014 Research-status Report
  • [Presentation] 核ラミナ構成タンパク質CRWNによる遺伝子発現制御機構の解析2018

    • Author(s)
      坂本勇貴、高木慎吾、松永幸大
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナの向背軸変異体における葉緑体光定位運動2018

    • Author(s)
      小島崇裕、石田泰浩、高木慎吾
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アズキ上胚軸の回旋運動と植物ホルモンの分布II2017

    • Author(s)
      飯田幹之、松浦恭和、森泉、高木慎吾
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 核ラミナタンパク質CRWNによる遺伝子発現制御メカニズムの解析2017

    • Author(s)
      坂本勇貴、高木慎吾、松永幸大
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] オルガネラ運動におけるシロイヌナズナミオシンXI-Iの役割2017

    • Author(s)
      宮武ゆう子、段中瑞、中野明彦、高木慎吾、伊藤光二、富永基樹
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナ単離葉緑体におけるアクチン構築機構の解析2016

    • Author(s)
      甲斐卓、森井真美、高木慎吾
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] CRWNは核ラミナタンパク質か?2016

    • Author(s)
      坂本勇貴、佐藤繭子、佐藤裕太、豊岡公徳 、高木慎吾、松永幸大
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 苔類ゼニゴケにおける細胞核光定位運動2016

    • Author(s)
      岩渕功誠、小松愛乃、酒井友希、田村謙太郎、高木慎吾、河内孝之、西村いくこ
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] シロイヌナズナにおける青色光依存的なミトコンドリアの運動促進とミオシンXI-iの関与について2016

    • Author(s)
      大西厚輝、Islam MS、富永基樹、高木慎吾
    • Organizer
      2016生体運動研究合同班会議
    • Place of Presentation
      京都
    • Year and Date
      2016-01-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 葉緑体アンカーにおけるビリンの役割2015

    • Author(s)
      高木慎吾
    • Organizer
      植物細胞骨格研究会2015
    • Place of Presentation
      三島
    • Year and Date
      2015-11-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulation of mitochondrial behavior by phototropins, photosynthesis, and myosin XI-I in Arabidopsis thaliana2015

    • Author(s)
      Islam MS, Nguyen VT, Tominaga M, Kato Y, Sakamoto W, Takagi S
    • Organizer
      International Microscopy Workshop on Plant Sciences
    • Place of Presentation
      Himeji
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Functions of phototropins and photosynthesis in the light induced mitochondria chloroplasts co-localization in Arabidopsis thaliana2015

    • Author(s)
      Islam MS, Nguyen VT, Kato Y, Sakamoto W, Takagi S
    • Organizer
      The 2nd East-Asia Microscopy Conference
    • Place of Presentation
      Himeji
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Role of proton motive force in red-light-induced cytoplasmic streaming in Vallisneria mesophyll cells2015

    • Author(s)
      Harada A, Okazaki Y, Kinoshita T, Nagai R, Takagi S
    • Organizer
      EWRS Aquatic Plants Meeting
    • Place of Presentation
      Edinburgh UK
    • Year and Date
      2015-09-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ホウレンソウのビリン様蛋白質が葉緑体アンカーに果たす役割2015

    • Author(s)
      高松秀安、森井真美、横田悦雄、高木慎吾
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] シロイヌナズナにおける核ラミナタンパク質CRWNの解析2015

    • Author(s)
      坂本勇貴、佐藤繭子、豊岡公徳 、高木慎吾、松永幸大
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物の細胞核の自然環境下における定位運動とその生理学的役割2015

    • Author(s)
      岩渕功誠、日出間純、田村謙太郎、高木慎吾、工藤洋、西村いくこ
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] シロイヌナズナにおける核の形態制御にかかわるCRWNsの解析2015

    • Author(s)
      坂本勇貴、杉山智哉、高木慎吾、松永幸大
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] アクチン結合蛋白質ビリンの活性はリン脂質により修飾される2015

    • Author(s)
      横田悦雄、織井秀文、田原寛、森安裕二、新免輝男、高木慎吾
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Regulation and significance of mitochondria-chloroplasts co-localization in Arabidopsis thaliana2015

    • Author(s)
      Islam MS, Nguyen VT, Kato Y, Sakamoto W, Takagi S
    • Organizer
      Tokyo Tech-HHU Düsseldorf Joint Symposium on Photosynthesis as a New Chemical Resource
    • Place of Presentation
      東京
    • Year and Date
      2015-03-04 – 2015-03-05
    • Related Report
      2014 Research-status Report
  • [Presentation] CROWDED NUCLEI regulates nuclear morphology in Marchantia polymorpha2014

    • Author(s)
      Kudo H, Sakamoto Y, Ishida S, Nishihama R, Kohchi T, Takagi S
    • Organizer
      Marchantia Workshop 2014
    • Place of Presentation
      神戸
    • Year and Date
      2014-12-08 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 植物特異的核タンパク質CRWNによる核構造制御機構の解析2014

    • Author(s)
      坂本勇貴、杉山智哉、高木慎吾、松永幸大
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナ葉肉細胞の核は弱光定位運動を示す2014

    • Author(s)
      小畑響子、田中怜、高木慎吾
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナにおける青色光に依存したミトコンドリアの移動速度調節について2014

    • Author(s)
      Islam MS、Nguyen VT、富永基樹、高木慎吾
    • Organizer
      第18回日本光生物学協会年会
    • Place of Presentation
      大阪
    • Year and Date
      2014-08-22 – 2014-08-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞核の形態維持に関与するシロイヌナズナCRWNについて2014

    • Author(s)
      坂本勇貴、杉山智哉、松永幸大、高木慎吾
    • Organizer
      第3回近畿植物学会講演会
    • Place of Presentation
      交野
    • Year and Date
      2014-04-19
    • Related Report
      2014 Research-status Report
  • [Remarks] 植物細胞生物学研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2015 Research-status Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2021-03-11  

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