| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Steroidal glycoalkaloid (SGA) alpha-tomatine with anti-nutritional activity, accumulates in tomato. Tomato genes encoding Jasmonate-Responsive Etheylene Response Factors (JREs), mostly clustered on chromosome I, were induced with methyl jasmonate treatment. Expression of SGA biosynthesis genes and accumulation of SGAs were markedly increased and decreased by overexpression and dominant suppression of JREs, respectively, implicating that JREs positively regulate SGA biosynthesis genes at transcript level. Transient transactivation assay in tomato fruits demonstrated that JREs activated the promoters of SGA biosynthetic DWF5 and GAME4 genes. The JRE-dependent activation of the promoters were clearly cancelled by mutations at JRE-binding sites found in proximal upstream regions of the genes. Putative JRE-binding elements predicted in promoter regions were found to be enriched significantly among a series of JRE-regulated genes.
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