Transcription factors regulating alkaloid biosynthesis in plants
Project/Area Number |
26440144
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Shoji Tsubasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (40343272)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | ステロイドアルカロイド / トマト / 転写因子 / アルカロイド / グルコアルカロイド / ジャスモン酸 |
Outline of Final Research Achievements |
Steroidal glycoalkaloid (SGA) alpha-tomatine with anti-nutritional activity, accumulates in tomato. Tomato genes encoding Jasmonate-Responsive Etheylene Response Factors (JREs), mostly clustered on chromosome I, were induced with methyl jasmonate treatment. Expression of SGA biosynthesis genes and accumulation of SGAs were markedly increased and decreased by overexpression and dominant suppression of JREs, respectively, implicating that JREs positively regulate SGA biosynthesis genes at transcript level. Transient transactivation assay in tomato fruits demonstrated that JREs activated the promoters of SGA biosynthetic DWF5 and GAME4 genes. The JRE-dependent activation of the promoters were clearly cancelled by mutations at JRE-binding sites found in proximal upstream regions of the genes. Putative JRE-binding elements predicted in promoter regions were found to be enriched significantly among a series of JRE-regulated genes.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Jasmonate-responsive ERF transcription factors regulate steroidal glycoalkaloid biosynthesis in tomato.2016
Author(s)
Chonprakun Thagun1, Shunsuke Imanishi2, Toru Kudo3, Ryo Nakabayashi4, Kiyoshi Ohyama5, Tetsuya Mori4, Koichi Kawamoto6, Yukino Nakamura3, Minami Katayama3, Satoko Nonaka6, Chiaki Matsukura6, Kentaro Yano3, Hiroshi Ezura6, Kazuki Saito4, Takashi Hashimoto1, and Tsubasa Shoji1
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Journal Title
Plant & Cell Physiology
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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