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Analysis of signaling of CLV3 and CLEs in Arabidopsis thaliana

Research Project

Project/Area Number 26440151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKumamoto Health Science University (2017)
Kumamoto University (2014-2016)

Principal Investigator

Yamaguchi Yasuka  熊本保健科学大学, 保健科学部, 研究員 (90448522)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsCLE / CLV3 / シロイヌナズナ / ペプチドホルモン / CLE遺伝子 / 植物器官形成
Outline of Final Research Achievements

The CLE gene family shows a wide distribution and functional duplication in various organs, and some of them show important functions in the plant world. At the beginning of the research program, we focused only on the CLV3 signal pathway, which regulates cell proliferation in the shoot apex. However, it is expected to work complementarily since the CLE gene group has similar expression patterns and peptide sequences, so, it is hardly difficult to clarify the functions of these CLE gene pathway without each CLE gene mutants. Therefore, by using the CRISPR method, we established all 32 CLE gene deficient resources. In addition, we found new phenotypes of the double mutant of CLE 41 and CLE 44. These results were published in 2017 issue of Plant and Cell Physiology Journal.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (4 results)

  • [Journal Article] A Collection of Mutants for CLE-Peptide-Encoding Genes in Arabidopsis Generated by CRISPR/Cas9-Mediated Gene Targeting2017

    • Author(s)
      Yamaguchi Yasuka L、Ishida Takashi、Yoshimura Mika、Imamura Yuko、Shimaoka Chie、Sawa Shinichiro
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 11 Pages: 1848-1856

    • DOI

      10.1093/pcp/pcx139

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CLE peptides and their signaling pathways in plant development.2016

    • Author(s)
      Yamaguchi, Y., Ishida, T.,Sawa, S.
    • Journal Title

      J. Exp. Bot.

      Volume: 67 Issue: 16 Pages: 4813-4826

    • DOI

      10.1093/jxb/erw208

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sall4 is essential for mouse primordial germ cell specification by suppressing somatic cell program genes.2015

    • Author(s)
      Yamaguchi YL, Tanaka SS, Kumagai M, Fujimoto Y, Terabayashi T, Matsui Y, Nishinakamura R.
    • Journal Title

      Stem Cells.

      Volume: 33(1) Issue: 1 Pages: 289-300

    • DOI

      10.1002/stem.1853

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] シロイヌナズナCLE16およびCLE17の機能解析2017

    • Author(s)
      島岡知恵、山口泰華、澤進一郎、石田喬志
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR/spCas9システムを利用したCLEペプチドの機能欠失変異体コレクションの作出2016

    • Author(s)
      田中(山口)泰華、吉村美香、今村悠子、島岡知恵、澤進一郎、石田喬志
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Generation of CRISPR/spCas9 mediated loss-of-CLE peptide mutants in Arabidopsis.2016

    • Author(s)
      Yasuka Yamaguchi, Mika Yoshimura, Yuko Imamura, Chie Shimaoka, Ryota Tateishi, Shinichiro Sawa, Takashi Ishida.
    • Organizer
      第57回日本植物生理学会
    • Place of Presentation
      岩手大学 上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Root-knot nematodes utilize plant CLE signaling for infection process2014

    • Author(s)
      Yamaguchi YL, Yumi Kanemaru, Chika Ejima, Shinichiro Sawa.
    • Organizer
      JSDB-APDBN
    • Place of Presentation
      WINK AICHI
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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