Analysis on molecular mechanism of protein transport to peroxisomes
Project/Area Number |
26440157
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | National Institute for Basic Biology |
Principal Investigator |
Mano Shoji 基礎生物学研究所, 多様性生物学研究室, 准教授 (20321606)
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Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA MIKIO 基礎生物学研究所, 名誉教授 (80093061)
KANAI MASATAKE 基礎生物学研究所, 多様性生物学研究室, NIBBリサーチフェロー (30611488)
OIKAWA KAZUSATO 理化学研究所, 環境資源科学研究センター, 研究員 (70508457)
KAMIGAKI AKANE 基礎生物学研究所, 多様性生物学研究室, 特別協力研究員 (30399315)
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Research Collaborator |
KONDO MAKI
GOTO SHINO
HIKINO KAZUMI
NAGATA KYOKO
YAMAGUCHI CHINAMI
NAKAGAWA TSUYOSHI
KOUCHI TAKAYUKI
NISHIHAMA RYUICHI
YAMATO KATSUYUKI
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | ペルオキシソーム / シロイヌナズナ / apem変異体 / タンパク質輸送 / Peroxin (PEX) / GFP / オルガネラ / ゼニゴケ / Peroxin / ユビキチン / タンパク質間相互作用 / タンパク質相互作用 |
Outline of Final Research Achievements |
From imaging analysis using transgenic Marchantia polymorpha expressing PTS1 (Peroxisome targeting signal 1)- or PTS2-Citrine and immunoelectron microscopic analysis revealed the presence of PTS1- and PTS2-dependent protein transport in M. polymorpha. Bioinformatics analysis identified the orthologous genes in M. polymporpha genome. Using this information, we succeeded in generation of some mutants whose peroxisomal genes were disrupted by the CRISPR/Cas9 method. In addition, we generated the Gateway technology-compatible binary vectors, and these vectors functioned in M. polymorpha cells. From the analysis of one of Arabidopsis apem (aberrant peroxisome morphology), the ubiquitin signaling pathway is involved in protein transport to peroxisomes, and that the defective of ubiquitin-dependent protein transport causes normal peroxisome functions, such as lipid metabolism and photorespiration.
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Report
(5 results)
Research Products
(36 results)
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[Journal Article] A dual-site gateway cloning system for simultaneous cloning of two genes for plant transformation.2017
Author(s)
Aboulela, M., Tanaka, Y., Nishimura, K., Mano, S., Kimura, T., and Nakagawa, T.
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Journal Title
Plasmid
Volume: 92
Pages: 1-11
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research.2017
Author(s)
Aboulela, M., Tanaka, Y., Nishimura, K., Mano, S., Nishimura, M., Ishiguro, S., Kimura, T., and Nakagawa, T.
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Journal Title
PLoS One
Volume: 12
Issue: 5
Pages: 177889-177901
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sucrose production mediated by lipid metabolism suppresses physiological interaction of peroxisomes and oil bodies during germination of Arabidopsis thaliana.2016
Author(s)
Cui, S., Hayashi, Y., Otomo, M., Mano, S., Oikawa, K., Hayashi, M. and Nishimura, M.
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Journal Title
J. Biol. Chem.
Volume: 291
Issue: 38
Pages: 19734-19745
DOI
Related Report
Peer Reviewed
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[Journal Article] Quantification of the adhesion strength between peroxisomes and chloroplasts by femtosecond laser technology2016
Author(s)
Hosokawa, Y., Iino, T., Oikawa, K., Mano, S., Yamada, K., and Nishimura, M.
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Journal Title
Bio-Protoc.
Volume: 6
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis.2016
Author(s)
Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
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Journal Title
Plant Physiol.
Volume: 170
Issue: 2
Pages: 867-880
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Dynamics of the light-dependent transition of plant peroxisomes.2015
Author(s)
Goto-Yamada, S., Mano, S., Yamada, K., Oikawa, K., Hosokawa, Y., Hara-Nishimura, I., and Nishimura, M.
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Journal Title
Plant and Cell Physiology
Volume: 56
Issue: 7
Pages: 1252-1263
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis2015
Author(s)
Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa & Mikio Nishimura
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Journal Title
Nature Plants
Volume: 1
Issue: 4
Pages: 15035-15035
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Development of two Gateway binary vector series for the assembly of three DNA fragments and promoter analysis in Marchantia polymorpha2017
Author(s)
Mano, S., Nishihama, R., Ishida, S., Kazumi, H., Kondo, M., Yamato, K., Kohchi, T., and Nakagawa, T.
Organizer
The 65th NIBB Conference Marchantia Workshop 2017 Renaissance of Marchantia polymorpha - the genome and beyond
Related Report
Int'l Joint Research
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[Presentation] Dynamics of peroxisomes and oil bodies based on imaging approach: Molecular players, mechanisms, and roles in metabolisms2016
Author(s)
Mano, S., Oikawa, K., Goto-Yamada, S., Shibata, M., Cui, S., Hayashi, M., and Nishimura, M.
Organizer
第57回日本植物生理学会年会
Place of Presentation
岩手大学(岩手県・盛岡市)
Year and Date
2016-03-18
Related Report
Invited
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[Presentation] Chaperone and protease functions of LON protease 2 modulate the peroxisomal transition and degradation with autophagy2016
Author(s)
Goto-Yamada, S., Mano, S., Nakamori, C., Kondo, M., Yamawaki, R, Kato, A., and Nishimura, M.
Organizer
第57回日本植物生理学会年会
Place of Presentation
岩手大学(岩手県・盛岡市)
Year and Date
2016-03-18
Related Report
Invited
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[Presentation] Photosynthesis-dependent physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis2015
Author(s)
Oikawa, K., Matsunaga, S., Mano, S., Kondo, M., Hayashi, M., Sakamoto, W., Mitsui, T., Iino, T., Hosokawa, Y., and Nishimura, M.
Organizer
The 2nd International Symposium on Plant Environmental Sensing
Place of Presentation
東京農業大学(東京都)
Year and Date
2015-03-13 – 2015-03-15
Related Report
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