Regulation of plasmodesmata in brown algae

• Project/Area Number: 26440160
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Hokkaido University
• Principal Investigator: NAGASATO Chikako 北海道大学, 北方生物圏フィールド科学センター, 准教授 (00374710)
• Co-Investigator(Kenkyū-buntansha): 田中 厚子 北海道大学, 北方生物圏フィールド科学センター, 特任助教 (40509999)
• Research Collaborator: FUJITA Tomomichi 北海道大学, 大学院理学研究院, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 褐藻類 / 原形質連絡 / 発生 / 微細構造 / マイクロインジェクション / 排除分子量 / 分化

Outline of Final Research Achievements

Brown algae show multicellularity and possess cellular connections called plasmodesmata which enable exchange of molecules between the neighboring cells. Plasmadesmata take tubular structure with 10 - 20 nm in diameter, and vary appearance and distribution with the developmental stages. Some new findings concerning brown algal plasmodesmata were given in this study as follows; 1) plasmodesmata are divided into primary and secondary plasmodesmata as in land plants, 2) plasmodesmatal size exclusion limit are set between 20-40 kDa in multiseriate species, and 3) pit fields in which plasmodesmata concentrate one place on the cross wall pass molecules less than 10 kDa to the next cell.

Research Products

• [Int'l Joint Research] アテネ大学(ギリシャ)
• [Int'l Joint Research] アテネ大学(ギリシャ)
• [Journal Article] Branch regeneration induced by sever damage in the brown alga Dictyota dichotoma (Dictyotales, Phaeophyceae). (2017)
  • Author(s): Tanaka, A., Hoshino, Y., Nagasato, C. and Motomrua, T.
  • Journal Title: Protoplasma Volume: 254 Issue: 3 Pages: 1341-1351
  • DOI: 10.1007/s00709-016-1025-4
  • Peer Reviewed
• [Journal Article] Intercellular translocation of molecules via plasmodesmata in the multiseriate filamentousbrown alga, Halopteris congesta (Sphacelariales,Phaeophyceae). (2017)
  • Author(s): Nagasato, C, Tanaka, A., Ito, T., Katsaros, C. and Motomura T.
  • Journal Title: Journal of Phycology Volume: 53 Issue: 2 Pages: 333-341
  • DOI: 10.1111/jpy.12498
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 多細胞藻類における一次細胞間連絡の形成 (2016)
  • Author(s): 長里千香子・寺内真・本村泰三
  • Journal Title: 顕微鏡 Volume: 51 Pages: 140-144
  • NAID: 130007695986
  • Peer Reviewed
• [Journal Article] Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus. (2016)
  • Author(s): Kinoshita N, Shiba K, Inaba K, Fu G, Nagasato C and Motomura T.
  • Journal Title: European Journal of Phycology Volume: 51 Issue: 2 Pages: 139-148
  • DOI: 10.1080/09670262.2015.1109144
  • NAID: 120006220013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mitotic spindle formation in Triparma laevis NIES-2565 (Parmales, Heterokontophyta). (2016)
  • Author(s): Yamada, K., Nagasato, C., Motmomura, T., Ichinomiya, M., Kuwata, A., Kamiya, M., Ohki, K. & Yoshikawa, S.
  • Journal Title: Protoplasma Volume: 未定 Issue: 1 Pages: 461-471
  • DOI: 10.1007/s00709-016-0967-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chemotactic movement in sperm of the oogamous brown algae, Saccharina japonica and Fucus distichus (2016)
  • Author(s): Kinoshita N, Nagasato C and Motomura T.
  • Journal Title: Protoplasma Volume: 55
  • Peer Reviewed
• [Journal Article] Chemotaxis in the anisogamous brown alga Mutimo cylindricus (2016)
  • Author(s): Kinoshita N, Nagasato C, Tanaka A, Motomura T.
  • Journal Title: Phycologia Volume: 55
  • Peer Reviewed
• [Journal Article] Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae) (2016)
  • Author(s): Terauchi M, Nagasato C, Inoue A, Ito T and Motomura T.
  • Journal Title: Planta Volume: 243 Issue: 2 Pages: 361-377
  • DOI: 10.1007/s00425-016-2516-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development and function of plasmodesmata in Fucus zygotes. (2015)
  • Author(s): Nagasato C, Terauchi M, Tanaka A and Motomura T.
  • Journal Title: Botanica Marina Volume: 58 Issue: 3 Pages: 229-238
  • DOI: 10.1515/bot-2014-0082
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phylogeographic data revealed shallow genetic structure in the kelp Saccharina japonica (Laminariales, Phaeophyta). (2015)
  • Author(s): Zhang J, Yao JT, Sun ZM, Fu G, Galanin DA, Nagasato C, Motomura T, Hu ZM and Duan DL.
  • Journal Title: BMC Evolutionary Biology Volume: 15 Issue: 1 Pages: 1-12
  • DOI: 10.1186/s12862-015-0517-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plasmodesmata of brown algae. (2015)
  • Author(s): Terauchi, M, Nagasato, C, and Motomura, T.
  • Journal Title: J. Plant Res. Volume: 128 Issue: 1 Pages: 7-15
  • DOI: 10.1007/s10265-014-0677-4
  • Peer Reviewed
• [Journal Article] Spermatogenesis and auxospore structure in the multipolar centric diatom Hydrosera (2015)
  • Author(s): Masahiko Idei, Shinya Sato, Chikako Nagasato, Taizo Motomura, Kensuke Toyoda, Tamotsu Nagumo, David G. Mann
  • Journal Title: Journal of Phycology Volume: 51 Issue: 1 Pages: 144-158
  • DOI: 10.1111/jpy.12261
  • Peer Reviewed
• [Journal Article] Proteomics analysis of heterogeneous flagella in brown alga (Stramenopiles) (2014)
  • Author(s): Fu, G., Nagasato, C., Oka, S., Cock, M. J. and Motomura, T.
  • Journal Title: Protist Volume: 165 Issue: 5 Pages: 662-675
  • DOI: 10.1016/j.protis.2014.07.007
  • NAID: 120005906760
  • Peer Reviewed
• [Journal Article] Identification of three proteins involved in fertilization and parthenogenetic development of a brown alga, Scytosiphon lomentaria (2014)
  • Author(s): Jong Won Han, J. W., Tatyana A. Klochkova, T. A, Shim, J., Nagasato, C. Motomura, T., and Kim, G. H.
  • Journal Title: Planta Volume: 240 Issue: 6 Pages: 1253-1267
  • DOI: 10.1007/s00425-014-2148-5
  • Peer Reviewed
• [Presentation] 褐藻Halopteris congestaにおける原形質連絡の構造と排除分子量の解析 (2015)
  • Author(s): 長里千香子、田中厚子、Christos Katsaros、本村泰三
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-09-07
• [Presentation] マイクロインジェクションを用いた褐藻類の原形質連絡における排除分子量の解析 (2015)
  • Author(s): 長里千香子・田中厚子・Christos Katsarost・本村泰三
  • Organizer: 日本藻類学会第39回福岡大会
  • Place of Presentation: 九州大学（福岡県・福岡市）
  • Year and Date: 2015-03-22
• [Presentation] Development and function of plasmodesmata in Fucus zygotes. (2014)
  • Author(s): Chikako Nagasato, Atsuko Tanaka, Martina Strittmatter, Susana Coelho, Mark Cock, Taizo Motomura
  • Organizer: 7th Asian Pacific Phycological Forum
  • Place of Presentation: Wuhan (中国）
  • Year and Date: 2014-09-21