Osmotic stress sensing and angiotensin II in body fluids homeostasis in amphibian CNS
Project/Area Number |
26440164
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | University of Toyama |
Principal Investigator |
UCHIYAMA Minoru 富山大学, 大学院理工学研究部(理学), 客員教授 (50095072)
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Co-Investigator(Kenkyū-buntansha) |
今野 紀文 富山大学, 大学院理工学研究部(理学), 講師 (50507051)
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Research Collaborator |
MUKUDA Takao
HARUMI Tatsuo
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 両生類 / 体液恒常性 / 脳室周囲器官 / c-Fos / アンギオテンシン受容体 / 無尾両生類 / アンギオテンシンⅡ / AT1R免疫陽性ニューロン / c-Fos免疫陽性ニューロン / TRPVファミリー / ATR / 血管平滑筋 / c-fosタンパク / 吸水行動 / アンギオテンシンII |
Outline of Final Research Achievements |
The present study aimed to investigate forebrain areas and role of angiotensin II in body fluid homeostasis in amphibian brain. Circumventricular organs lack blood-brain barrier and probably function to detect changes of extracellular fluid. Although we cloned amphibian transient receptor potential vanilloid (TRPV) 1 and TRPV4 activated Ca++ permeability in response to osmotic stress in vitro, there was no significant change of TRPV-4 mRNA expression in dehydrated frogs using real-time quantitative PCR. C-Fos immunoreactive neurons were observed in several nuclei including the paraventricular nucleus (PVN) in forebrain of Japanese tree frog. The number of c-Fos immunoreactive cells increased in these nuclei of frogs under dehydrated condition. Angiotensin II type 1 receptor was immunohistochemically located in the medial septal nucleus (MSN). There may be physiological relationship between MSN and paraventricular nucleus according to analysis of the expression of c-Fos protein.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Urotensin II upregulates migration and cytokine gene expression in leukocytes of the African clawed frog, Xenopus laevis.2015
Author(s)
Tomiyama, S., Nakamachi, T., Uchiyama, M., Matsuda, K., and Konno, N.
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Journal Title
Gen. Comp. Endocrinol.
Volume: in press
Pages: 54-63
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Ghrelin receptor in amphibians2014
Author(s)
Hiroyuki Kaiya, Takio Kitazawa, Norifumi Konno, Minoru Uchiyama, Kenji Kangawa and Mikiya Miyazato
Organizer
International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
Place of Presentation
National Institute for Basic Biology, Okazaki, Japan
Year and Date
2014-11-08
Related Report
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