| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Tapetum and male gametophyte in higher plants contains characteristic lipid-rich organelles. Transmission electron microscopy (TEM) is a powerful method for the ultrastructural observation of organelles. To understand the mechanism for formation of these lipid-rich organelles in tapetum and male gametophyte, I made an organelle-map using transmission electron microscopy in wild-type Arabidopsis. Digital TEM images were captured by a custom auto-acquisition system and merged by an auto-image tiling program. Furthermore, I analyzed the Arabidopsis mutants of lipid metabolism and described the role of lipids on the male gametophyte and tapetum development. It is becoming clear that some lipids are critical for tapetal lipid-rich organelles.
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