Wide-range ultrastructural analysis of organelles in pollen formation
Project/Area Number |
26440170
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
|
Research Institution | Japan Women's University |
Principal Investigator |
Nagata Noriko 日本女子大学, 理学部, 教授 (40311352)
|
Research Collaborator |
KOBAYASHI Megumi
HONDA Tamami
SAWAKI Fumie
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 電子顕微鏡 / オルガネラ / 脂質 / 葯 / 花粉 / シロイヌナズナ |
Outline of Final Research Achievements |
Tapetum and male gametophyte in higher plants contains characteristic lipid-rich organelles. Transmission electron microscopy (TEM) is a powerful method for the ultrastructural observation of organelles. To understand the mechanism for formation of these lipid-rich organelles in tapetum and male gametophyte, I made an organelle-map using transmission electron microscopy in wild-type Arabidopsis. Digital TEM images were captured by a custom auto-acquisition system and merged by an auto-image tiling program. Furthermore, I analyzed the Arabidopsis mutants of lipid metabolism and described the role of lipids on the male gametophyte and tapetum development. It is becoming clear that some lipids are critical for tapetal lipid-rich organelles.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Platform for “Chemical Metabolic Switching” to Increase Sesquiterpene Content in Plants2017
Author(s)
K. Kobayashi, K. Kobayashi, H. Yamaguchi, Y. M. Inoue, K. Takagi, K. Fushihara, H. Seki, M. Suzuki, N. Nagata, T. Muranaka
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Journal Title
Plant Biotechnology
Volume: 34
Issue: 1
Pages: 65-69
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed / Open Access
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[Journal Article] Modification of plant cell wall structure accompanied by enhancement of saccharification efficiency using a chemical, lasalocid sodium2016
Author(s)
Okubo-Kurihara, E., Ohtani, M., Kurihara, Y., Kakegawa, K., Kobayashi, M., Nagata, N., Komatsu, T., Kikuchi, J., Cutler, S., Demura,T.and Matsui, M.
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 34602-34602
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated apoplastic space in Arabidopsis leaf epidermis2016
Author(s)
Akita, K., Kobayashi, M., Sato, M., Kutsuna, N., Ueda, T., Toyooka, K., Nagata, N., Hasezawa, S., and Higaki, T.
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Journal Title
Protoplasma
Volume: in press
Issue: 1
Pages: 367-377
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i>2016
Author(s)
Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H.
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Journal Title
CYTOLOGIA
Volume: 81
Issue: 1
Pages: 69-76
DOI
NAID
ISSN
0011-4545, 1348-7019
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Plastid Proteomic Analysis in Tomato Fruit Development.2015
Author(s)
M. Suzuki, S. Takahashi, T. Kondo, H. Dohra, Y. Ito, Y. Kiriiwa, M. Hayashi, S. Kamiya, M. Kato, M. Fujiwara, Y. Fukao, M. Kobayashi, N. Nagata, R. Motohashi
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Journal Title
PLOS ONE
Volume: 15; 10(9)
Issue: 9
Pages: e0137266-e0137266
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Integrated analysis of transcriptome and metabolome of Arabidopsis albino or pale green mutants with disrupted nuclear-encoded chloroplast proteins.2014
Author(s)
M. Satou, H. Enoki, A. Oikawa, D. Ohta, K. Saito, T. Hachiya, H. Sakakibara, M. Kusano, A. Fukushima, K. Saito, M. Kobayashi, N. Nagata, F. Myouga, K. Shinozaki, R. Motohashi
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Journal Title
Plant Molecular Biology
Volume: 85
Issue: 4-5
Pages: 411-428
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Challenge to Organellomics by the Transmission Electron Microscopy.2015
Author(s)
N. Nagata, A. Kato, F. Sawaki, M. Kobayashi, M. Sato, T. Higaki, N. Kutsuna, S. Hasezawa, F. Myouga and K. Toyooka
Organizer
The 2nd International Symposium on Plant Environmental Sensing
Place of Presentation
National Institute of Advanced Industrial Science and Technology, Tokyo, Japan
Year and Date
2015-03-13 – 2015-03-15
Related Report
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