| Project/Area Number |
26440174
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Kaiya Hiroyuki 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (40300975)
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| Co-Investigator(Kenkyū-buntansha) |
宮里 幹也 国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50291183)
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| Co-Investigator(Renkei-kenkyūsha) |
KANGAWA Kenji 国立研究開発法人国立循環器病研究センター研究所, 所長 (00112417)
KONNO Norifumi 富山大学, 理工学部, 講師 (50507051)
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| Research Collaborator |
HIRAOKA Kiyoshi 株式会社フジキン
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | グレリン / GHS-R / 機能解析 / 摂食量 / 浸透圧調節 / エネルギー代謝 / 遺伝子発現 / GHS-R1a / グレリン受容体 / 両生類 / アホロートル / イベリアトゲイモリ / チョウザメ / 発生 / 定量的PCR / 脳 / 下垂体 / 肺魚 / ツメガエル |
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| Outline of Final Research Achievements |
We determined the nucleotide sequence of the ghrelin precursor of a sturgeon (vestel) and structure of the mature ghrelin, and noticed that two kinds of mature ghrelin were produced from different mRNAs in the same individuals. In addition, we clarified that expression of the ghrelin gene increases as the development progresses from fertilized egg. Furthermore, we identified a functional ghrelin receptor (GHS-R) in the African lungfish, and it expresses in the brain, heart and intestines, and suggested the possibility to participate in osmoregulation and energy metabolism during the estivation. Moreover, two kinds of GHS-R that we discovered in axolotls varied in tissue distribution and expression level, and it was suggested that there was plural GHS-Rs in amphibians. In the fire belly newt and the Western clawed frog, intraperitoneal injection of the ghrelin did not affect the food intake.
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