Neural basis of coding for odor direction by a single olfactory organ

• Project/Area Number: 26440175
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hokkaido University
• Principal Investigator: Nishino Hiroshi 北海道大学, 電子科学研究所, 助教 (80332477)
• Co-Investigator(Renkei-kenkyūsha): MATSUSHITA Atsuko 総合研究大学院大学, 先導科学研究科, 助教 (50450416)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 昆虫 / ナビゲーション / プルーム / 触角 / 投射ニューロン / キノコ体 / 感覚子 / 性フェロモン / 組織化 / 遅延繊維 / 触角葉 / トポグラフィー / 嗅感覚細胞 / 時間的符号化 / 潜時 / フェロモン / 匂いプルーム

Outline of Final Research Achievements

The distribution of odor filaments in a fluid is ever-changing. How animals read out such instantaneously changing signals in the central nervous system and locate an odor source is an enigma of neuroscience. Typical nocturnal animals, cockroaches are able to locate an odor source based solely on antennal input but not on visual cues. We found that axons of sex pheromone-receptive neurons are orderly represented in different regions of the macroglomerulus based on the three-dimensional soma locations. Our intracellular recordings further revealed that a single projection neuron with dendrites throughout in the macroglomerulus exhibits different temporal patterns to odors given from different directions across the longitudinal antenna.

Research Products

• [Journal Article] Complete identification of four giant interneuronssupplying mushroom body calyces in the cockroach Periplaneta americana (2017)
  • Author(s): Takahashi N., Katoh K., Watanabe H., Nakayama Y., Iwasaki M., Mizunami M., Nishino H.
  • Journal Title: Journal of Comparative Neurology Volume: 525 Issue: 1 Pages: 204-230
  • DOI: 10.1002/cne.24108
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Group-housed females promote production of asexual ootheca in American cockroaches (2017)
  • Author(s): Katoh K., Iwasaki M., Hosono S., Yoritsue A., Ochiai M., Mizunami M., Nishino H.
  • Journal Title: Zoological Letters Volume: 3 Issue: 1 Pages: 3-3
  • DOI: 10.1186/s40851-017-0063-x
  • NAID: 120005981462
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Status of and future research on thermosensory processing (2016)
  • Author(s): Mizunami M, Nishino H, Yokohari F,
  • Journal Title: Frontiers in Physiology Volume: 7 Pages: 150-150
  • DOI: 10.3389/fphys.2016.00150
  • NAID: 120005766749
  • Peer Reviewed / Open Access
• [Journal Article] Dopamine- and tyrosine hydroxylase-immunoreactive neurons in the brain of the American Cockroach, Periplaneta americana (2016)
  • Author(s): Hamanaka Y., Minoura R., Nishino H., Miura T., Mizunami M.
  • Journal Title: Plos One Volume: 11 Issue: 8 Pages: e0160531-e0160531
  • DOI: 10.1371/journal.pone.0160531
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chordotonal organs in hemipteran insects: unique peripheral structures but conserved central organization revealed by comparative neuroanatomy (2016)
  • Author(s): H. Nishino, H. Mukai, T. Takanashi
  • Journal Title: Cell Tisue Res. Volume: 366 Issue: 3 Pages: 549-572
  • DOI: 10.1007/s00441-016-2480-0
  • NAID: 120006366485
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Coarse topographic organization of pheromone-sensitive afferents from different antennal surfaces in the American cockroach. (2015)
  • Author(s): Nishino H, Watanabe H, Kamimura I, Yokohari F, Mizunami M.
  • Journal Title: Neurosci. Lett. Volume: 595 Pages: 35-40
  • DOI: 10.1016/j.neulet.2015.04.006
  • NAID: 120005952006
  • Peer Reviewed / Open Access
• [Journal Article] Toward elucidating diversity of neural mechanisms underlying insect learning. (2015)
  • Author(s): Mizunami M, Hamanaka Y, Nishino H.
  • Journal Title: Zool. Lett. Volume: 1 Issue: 1 Pages: 8-8
  • DOI: 10.1186/s40851-014-0008-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] カメムシは左右脚部の振動受容器が生む強度差比較により振動源に定位する (2017)
  • Author(s): 向井 裕美、西野 浩史、スカルス ニールス、高梨 琢磨
  • Organizer: 第61回日本応用動物昆虫学会大会
  • Place of Presentation: 東京農工大学（東京都、小金井市）
  • Year and Date: 2017-03-27
• [Presentation] ワモンゴキブリの単為生殖のメカニズム (2017)
  • Author(s): 田中 真史、加藤 巧、西野 浩史、大門 高明
  • Organizer: 第61回日本応用動物昆虫学会大会
  • Place of Presentation: 東京農工大学（東京都、小金井市）
  • Year and Date: 2017-03-27
• [Presentation] シロアリの触角葉糸球体構成のカースト間比較 (2017)
  • Author(s): 古賀 晴華、渡邉 英博、西野 浩史、北條 優、大村 和香子、横張 文男
  • Organizer: 第61回日本応用動物昆虫学会大会
  • Place of Presentation: 東京農工大学（東京都、小金井市）
  • Year and Date: 2017-03-27
• [Presentation] Male jewel bug localizes female calling vibrations: directional vibration sensing by chordotonal organs (2016)
  • Author(s): Mukai H., Nishino H., Skals N., Taknashi T.
  • Organizer: 第3回生物音響学会年次研究発表会
  • Place of Presentation: 伊良湖シーパーク & スパ（愛知県、田原市)
  • Year and Date: 2016-12-10
  • Int'l Joint Research
• [Presentation] A neuron coding odor direction (2016)
  • Author(s): Nishino H., Iwasaki M., Takahashi N., Katoh K., Matsushita M.
  • Organizer: The 87th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県、宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Two parallel coding strategies to process general odor in basal insects (2016)
  • Author(s): Watanabe H., Nishino H., Mizunami M., Yokohari F.
  • Organizer: The 87th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県、宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Similarity and variability of glomerular organizations of the antennal lobes in seven species of termites (2016)
  • Author(s): Koga H., Watanabe H., Nishino H., Hojo M., Ohmura W., Takanashi T., Yokohari F.
  • Organizer: The 87th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県、宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Complete identification of four calycal giant interneurons in an insect brain. (2016)
  • Author(s): Takahashi N., Katoh K., Watanabe H., Mizunami M., Nishino H.
  • Organizer: The 87th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県、宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Vibration sensitivity in cerambycid beetles and its potential for insect pest control (2016)
  • Author(s): Takanashi T., Sakamoto H., Skals N., Fukui S., Matsui Y., Koika T., Nishino H.
  • Organizer: The 87th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県、宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Activity of a pheromone-responsive projection neuron is inhibited by pheromonal stimulation to the contralateral antenna. (2016)
  • Author(s): Nishino H., Katoh K., Iwasaki M., Takahashi N., Watanabe H.
  • Organizer: JSCPB 2016 Tokyo
  • Place of Presentation: 玉川大学（東京都、町田市）
  • Year and Date: 2016-09-02
  • Int'l Joint Research
• [Presentation] Distribution of putative octopaminergic and tyraminergic neurons in a cockroach brain (2016)
  • Author(s): Terao K., Watanabe T., Aonuma H., NishinoH., Mizunami M.
  • Organizer: JSCPB 2016 Tokyo
  • Place of Presentation: 玉川大学（東京都、町田市）
  • Year and Date: 2016-09-02
  • Int'l Joint Research
• [Presentation] 7種のシロアリの触角葉糸球体構成の比較解析 (2016)
  • Author(s): 古賀 晴華、渡邉 英博、西野 浩史、北條 優、大村 和香子、高梨 琢磨、横張 文男
  • Organizer: 動物学会・植物学会・生態学会三学会合同鹿児島大会
  • Place of Presentation: 鹿児島大学（鹿児島県、鹿児島市）
  • Year and Date: 2016-05-28
• [Presentation] ゴキブリのグループ効果：単為生殖における卵鞘形成同調 (2016)
  • Author(s): 加藤 巧、岩﨑 正純、水波 誠、西野 浩史
  • Organizer: 日本昆虫学会第76回大会・第60回日本応用動物昆虫学会・合同大会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス（大阪府・堺市）
  • Year and Date: 2016-03-26
• [Presentation] Olfactory processing via temporally and spatially segregated parallel pathways in an insect brain. (2016)
  • Author(s): Watanabe H., Nishino H., Mizunami M., Yokohari F.
  • Organizer: Environmental Sensing and Animal Behavior（ESAB）
  • Place of Presentation: 東京大学駒場キャンパス（東京都、目黒区）
  • Int'l Joint Research
• [Presentation] Aggregation pheromone processing in a cockroach brain (2016)
  • Author(s): Katoh K., Takahashi N., Watanabe H., Mizunami M., Nishino H.
  • Organizer: JSCPB 2016 Tokyo
  • Place of Presentation: 玉川大学（東京都、町田市）
  • Int'l Joint Research
• [Presentation] Complete identification of four types of calycal giant interneurons in an insect brain (2015)
  • Author(s): 高橋 直美、加藤 巧、渡邉 英博、水波 誠、西野 浩史
  • Organizer: CompBiol 2015 広島大会
  • Place of Presentation: JMS アステールプラザ （広島県・広島市）
  • Year and Date: 2015-12-11
• [Presentation] Dormitory effect in cockroaches: synchronization of assexual ootheca production in females (2015)
  • Author(s): 加藤 巧、岩崎 正純、水波 誠、西野 浩史
  • Organizer: CompBiol 2015 広島大会
  • Place of Presentation: JMS アステールプラザ （広島県・広島市）
  • Year and Date: 2015-12-11
• [Presentation] 単一ニューロンによる匂いの方向検出 (2015)
  • Author(s): 西野浩史、岩﨑正純、加藤巧、高橋直美
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-17
• [Presentation] マツノマダラカミキリ弦音器官がもつセロトニン免疫陽性の細胞内微細構造 (2015)
  • Author(s): 渡邉 英博、西野 浩史、高梨 琢磨、横張 文男
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-17
• [Presentation] Sensing the structural architecture of odor plumes with a single antenna. (2014)
  • Author(s): Nishino H., Iwasaki M., Yoritsune M., Kamimura I., Mizunami M.
  • Organizer: 11th International Congress of Neuroethology
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2014-07-28 – 2014-08-11
• [Presentation] Temporal activity patterns of two different types of projection neurons revealed by simultaneous intracellular recordings in the cockroach. (2014)
  • Author(s): Watanabe H., Nishino H., Yokohari F.
  • Organizer: 11th International Congress of Neuroethology
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2014-07-28 – 2014-08-01
• [Remarks] 西野研究室
  • URL: http://www.es.hokudai.ac.jp/labo/nishino/
• [Remarks] 複雑系数理学研究分野 西野研究室
  • URL: http://www.es.hokudai.ac.jp/labo/nishino/