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Study on the mechanism of action of insect flight muscle using muscle fibers with exchanged protein components

Research Project

Project/Area Number 26440185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionJapan Synchrotron Radiation Research Institute

Principal Investigator

IWAMOTO Hiroyuki  公益財団法人高輝度光科学研究センター, 利用研究促進部門, 特別研究員 (60176568)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords昆虫飛翔筋 / X線回折 / シンクロトロン放射光 / メカノセンシング
Outline of Final Research Achievements

Insects can beat their wings at frequencies of up to 1000 times/s. This is due to the capacity of the flight muscle to undergo fast oscillations. To clarify which of the constituent proteins this property comes from, we developed techniques to exchange specific constituent proteins with exogenous ones obtained from other organisms. Here we succeeded in replacing the endogenous actin filaments of the flight muscle with those from rabbit skeletal muscle. X-ray diffraction studies indicated that the rabbit actin filaments were regenerated in the right position in the muscle with a right orientation, and that the endogenous myosin can form rigor complexes with the rabbit actin filaments.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Remarks (1 results)

  • [Journal Article] The earliest molecular response to stretch of insect flight muscle as revealed by fast X-ray diffraction recording.2017

    • Author(s)
      Iwamoto, H.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 42272-42272

    • DOI

      10.1038/srep42272

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] X-ray diffraction pattern from the flight muscle of Toxorhynchites towadensis reveals mosquito’s specific phylogenic position in Diptera.2015

    • Author(s)
      Iwamoto, H.
    • Journal Title

      Zool. Lett.

      Volume: 1 Issue: 1 Pages: 24-24

    • DOI

      10.1186/s40851-015-0024-1

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] X-ray diffraction from flight muscle with a headless myosin mutation: implications for interpreting reflection patterns.2014

    • Author(s)
      Iwamoto, H., Trombitas, K., Yagi, N., Suggs, J.A. and Bernstein, S.I.
    • Journal Title

      Front. Physiol. Striated Muscle Physiol.

      Volume: 5 Pages: 416-416

    • DOI

      10.3389/fphys.2014.00416

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] RECONSTRUCTION OF FUNCTIONAL INSECT FLIGHT MUSCLE FIBERS WITH RABBIT SKELETAL MUSCLE ACTIN2017

    • Author(s)
      Iwamoto, H.
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      New Orleans (USA)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] 超音波を発生する昆虫の筋肉の構造2017

    • Author(s)
      岩本裕之
    • Organizer
      2017年 生体運動研究合同班会議
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Reconstitution of functional insect flight- and rabbit skeletal hybrid muscle fibers as monitored by X-ray diffraction2016

    • Author(s)
      Iwamoto, H.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] シンクロトロン放射光X線の動物学への適用:昆虫飛翔筋の高速X線回折像記録2016

    • Author(s)
      岩本裕之
    • Organizer
      第22回国際動物学会・第87回日本動物学会 合同大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The timbal muscle of cicadas resembles flight muscle in structure and protein expression2016

    • Author(s)
      Iwamoto, H.
    • Organizer
      第22回国際動物学会・第87回日本動物学会 合同大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ultrafast X-ray diffraction movie from the flight muscle of alive bee during wing-beat.2016

    • Author(s)
      Iwamoto, H.
    • Organizer
      31st International Congress on High Speed Imaging and Photonics.
    • Place of Presentation
      (Osaka, JAPAN)
    • Year and Date
      2016-10-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高速X線回折ムービー記録による昆虫飛翔筋動作機構の解明2016

    • Author(s)
      岩本裕之
    • Organizer
      第56回生物物理若手の会 夏の学校
    • Place of Presentation
      支笏湖ユースホステル(北海道千歳市)
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] X線で探るセミの歌声の起源2016

    • Author(s)
      岩本裕之
    • Organizer
      2016年 生体運動合同班会議
    • Place of Presentation
      京都府京都市
    • Year and Date
      2016-01-08
    • Related Report
      2015 Research-status Report
  • [Presentation] X-RAY DIFFRACTION FROM INSECT FLIGHT MUSCLE FIBERS WITH EXCHANGED CONTRACTILE PROTEINS2015

    • Author(s)
      Iwamoto, H. and Yagi, N.
    • Organizer
      59th Biophysical Society Meeting
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2015-02-07 – 2015-02-11
    • Related Report
      2014 Research-status Report
  • [Presentation] High‐speed in vivo X‐ray video recording from live bees2015

    • Author(s)
      Iwamoto, H.
    • Organizer
      Insect Muscle 2015: Biomedical and Biophysical Perspectives
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2015-02-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 羽ばたいている昆虫の飛翔筋分子ダイナミクスをX線で眺める2015

    • Author(s)
      岩本裕之
    • Organizer
      神戸大学 先端融合科学シンポジウム
    • Place of Presentation
      神戸大学(兵庫県、神戸市)
    • Year and Date
      2015-01-19 – 2015-01-20
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 日本最大の蚊、トワダオオカの飛翔筋線維のX線回折2015

    • Author(s)
      岩本裕之・八木直人
    • Organizer
      2015年 生体運動研究合同班会議
    • Place of Presentation
      学習院大学(東京都、豊島区)
    • Year and Date
      2015-01-07 – 2015-01-09
    • Related Report
      2014 Research-status Report
  • [Presentation] X-ray diffraction from insect flight muscle with exchanged contractile proteins2014

    • Author(s)
      岩本裕之・八木直人
    • Organizer
      日本生物物理学会第52回年会
    • Place of Presentation
      札幌コンベンションセンター(北海道、札幌市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular mechanism of action of insect flight muscle2014

    • Author(s)
      Iwamoto, H.
    • Organizer
      International Workshop for Diamond Researchers on ‘Three Dimensional Development of Lab-Exchange Type Biomedical Science Research Consortium’
    • Place of Presentation
      鴨川セミナーハウス(千葉県、鴨川市)
    • Year and Date
      2014-09-14
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 収縮蛋白を交換した昆虫飛翔筋線維のX線回折2014

    • Author(s)
      岩本裕之・八木直人
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学(宮城県、仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Remarks] 昆虫の羽ばたきを起こす筋肉内の分子変化の精密測定に成功(プレスリリース)

    • URL

      http://www.spring8.or.jp/ja/news_publications/press_release/2017/170208/

    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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