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Mechanism of meiotic DNA double strand break formation in chromatin structure

Research Project

Project/Area Number 26440186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genetics/Chromosome dynamics
Research InstitutionChuo University

Principal Investigator

YAMADA Takatomi  中央大学, 理工学部, 助教 (30451850)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords減数分裂 / 相同組換え / クロマチン / DNA二重鎖切断
Outline of Final Research Achievements

Meiotic recombination is initiated by programmed DNA double strand breaks (DSBs) formed at hotspots. Using fission yeast as a model system, we have been studying how DSBs are formed in chromatin structure, and previously reported that acetylated histone H3 lysine 9 (H3K9ac) and the histone H3 lysine 4 methyltransferase Set1 are involved in the event. Here, I have extended our analysis by examining roles of H3K9ac and Set1 and factors cooperating with these factors.
We found that transcription around the model hotspot ade6-3049 promotes H3K9ac at this hotspot. This tendency may be universal, as previously reported data sets suggested that transcripts are found in hotspots-surrounding regions. Our analyses also found that Set1 and the histone H2A.Z activates DSB formation in distinct pathways, and that H2A.Z facilitates DSB formation by modulating chromosome compaction to help DSB-related factors bind to chromatin.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] The histone variant H2A.Z promotes initiation of meiotic recombination in fission yeast.2018

    • Author(s)
      Yamada S, Kugou K, Ding DQ, Fujita Y, Hiraoka Y, Murakami H, Ohta K, Yamada T.
    • Journal Title

      Nucleic Acids Res.

      Volume: 46 Issue: 2 Pages: 609-620

    • DOI

      10.1093/nar/gkx1110

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The conserved histone variant H2A.Z illuminates meiotic recombination initiation.2018

    • Author(s)
      Yamada S, Kugou K, Ding DQ, Fujita Y, Hiraoka Y, Murakami H, Ohta K, Yamada T.
    • Journal Title

      Current Genetics

      Volume: 印刷中 Issue: 5 Pages: 1015-1019

    • DOI

      10.1007/s00294-018-0825-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 生体内における減数分裂期組換えの開始制御2018

    • Author(s)
      山田貴富 村上浩士
    • Journal Title

      化学と生物

      Volume: 56 Pages: 262-271

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots.2017

    • Author(s)
      . Yamada, M. Okamura, H. Murakami, K.Ohta, T. Yamada
    • Journal Title

      Genetics

      Volume: in press Issue: 2 Pages: 801-809

    • DOI

      10.1534/genetics.116.197954

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Local potentiation of stress-responsive genes by upstream noncoding transcription2016

    • Author(s)
      Naomichi Takemata, Arisa Oda, Takatomi Yamada, Josephine Galipon, Tomoichiro Miyoshi, Yutaka Suzuki, Sumio Sugano, Charles S. Hoffman, Kouji Hirota, and Kunihiro Ohta
    • Journal Title

      Nucleic Acids Research

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Two fission yeast high mobility group box proteins in the maintenance of genomic integrity following doxorubicin insult.2015

    • Author(s)
      Tang MY, Guo H, Nguyen TT, Low LS, Jackson RA, Yamada T, Chen ES
    • Journal Title

      Gene

      Volume: 562(1) Issue: 1 Pages: 70-75

    • DOI

      10.1016/j.gene.2015.02.041

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 分裂酵母ヒストンH3K4メチル化酵素Set1複合体の減数分裂期組換えへの関与2017

    • Author(s)
      山田貴富 太田邦史 村上浩士
    • Organizer
      酵母遺伝学フォーラム第50回研究報告会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 減数分裂期組換え開始におけるクロマチン関連因子の機能2017

    • Author(s)
      山田貴富 山田真太郎 久郷和人 丁大橋 藤田侑里香 平岡泰 太田邦史 村上浩士
    • Organizer
      第35回染色体ワークショップ・第16回核ダイナミクス研究会 合同開催
    • Related Report
      2017 Annual Research Report
  • [Presentation] Histone H2A.Z promotes meiotic DNA double strand break formation in fission yeast.2016

    • Author(s)
      Shintaro Yamada, Kazuto Kugou, Yurika Fujita, Hiroshi Murakami, Kunihiro Ohta, and Takatomi Yamada
    • Organizer
      Meiosis Gordon Research Conference
    • Place of Presentation
      New London, NH, U.S.A.
    • Year and Date
      2016-06-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 分裂酵母ヒストンH2A.Zの減数分裂期組換え開始への関与2016

    • Author(s)
      山田真太郎,藤田侑里香,久郷和人,村上浩士,太田邦史,山田貴富
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会合同開催
    • Place of Presentation
      松島
    • Year and Date
      2016-01-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 分裂酵母の減数分裂期組換えホットスポットにおける転写と組換えの関係2016

    • Author(s)
      山田真太郎、岡村美佳、村上浩士、太田邦史、山田貴富
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      神戸
    • Related Report
      2016 Research-status Report
  • [Presentation] Histone H2A.Z regulates initiation of meiotic recombination in fission yeast.2015

    • Author(s)
      Shintaro Yamada, Yurika Fujita, Kazuto Kugou, Hiroshi Murakami, Kunihiro Ohta, and Takatomi Yamada
    • Organizer
      EMBO Meiosis Conference 2015
    • Place of Presentation
      Oxford, UK
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Histone H2A.Z promotes meiotic DNA double-strand breaks in fission yeast.2015

    • Author(s)
      Shintaro Yamada, Yurika Fujita, Kazuto Kugou, Hiroshi Murakami, Kunihiro Ohta, and Takatomi Yamada
    • Organizer
      The 8th International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2019-03-29  

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