Genetic Identification of a novel cell cycle checkpoint that monitors damages in mitochondrial dynamics
Project/Area Number |
26440188
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Genetics/Chromosome dynamics
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
Inoue Yoshihiro 京都工芸繊維大学, 応用生物学系, 准教授 (90201938)
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Co-Investigator(Kenkyū-buntansha) |
山口 政光 京都工芸繊維大学, 応用生物学系, 教授 (00182460)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | ショウジョウバエ / ミトコンドリア / 細胞周期 / ダイナミクス / 減数分裂 / ミトコンドリア動態 / 細部周期 |
Outline of Final Research Achievements |
Mitochondria are dynamic organelles that engage in repeated cycles of fusion and fission. It serves to intermix the contents among mitochondria and to maintain a quality of a mitochondria population in a cell. In this study, we showed that the meiotic entry of a primary spermatocyte is inhibited when sever damages in mitochondrial DNA occurred. Activation of CDK1 failed to be observed in the arrested cells. The nuclear to cytoplasm transport of cyclin B was perturbed in the cells. We revealed that there is a checkpoint mechanism that monitors a condition of mitochondria and arrests the meiotic cell cycle in the presence of their abnormalities, such as mitochondrial DNA damage. To avoid production of abnormal sperm, such a specific checkpoint mechanism has been possibly provided in germline cells but not in somatic cells in Drosophila.
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Report
(5 results)
Research Products
(122 results)
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[Journal Article] Overexpression of dJmj differentially affects intestinal stem cells and differentiated enterocytes.2018
Author(s)
Suong, D. N. A., Shimaji, K., Pyo, J.-H., Park, J.-S., Yoshida, H., Yoo, M. and Yamaguchi, M.
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Journal Title
Cellular Signalling
Volume: 42
Pages: 194-210
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Histone methyltransferase G9a is a key regulator of the starvation-induced behaviors in Drosophila melanogaster.2017
Author(s)
Shimaji, K., Tanaka, R., Maeda, T., Ozaki, M., Yoshida, H., Ohkawa, Y., Sato, T., Suyama, M. and Yamaguchi, M.
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 14763-14763
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] ④Moyu Goto, Narumi Toda, Kouhei Shimaji, Dang Ngoc-Anh Suong, Nicole Vo, Hiroshi Kimura, Hideki Yoshida, Yoshihiro H. Inoue and Masamitsu Yamaguchi.2016
Author(s)
Moyu Goto, Narumi Toda, Kouhei Shimaji, Dang Ngoc-Anh Suong, Nicole Vo, Hiroshi Kimura, Hideki Yoshida, Yoshihiro H. Inoue and Masamitsu Yamaguchi.
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Journal Title
Spermatogenesis.
Volume: 6
Issue: 3
Pages: e1232023-e1232023
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Knockdown of the drosophila FIG4 induces deficient locomotive behavior, shortening of motor neuron, axonal targeting aberration, reduction of life span and defects in eye development2015
Author(s)
Kyotani A, Azuma Y, Yamamoto I, Yoshida H, Mizuta I, Mizuno T, Nakagawa M, Tokuda T, Yamaguchi M
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Journal Title
Exp Neurol
Volume: 277
Pages: 86-95
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis2014
Author(s)
Kitazawa, D., Matsuo, T., Kaizuka, K., Miyauchi, C., Hayashi, D. and Inoue, Y. H. Orbit
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Journal Title
PLoS ONE
Volume: 9(5)
Issue: 5
Pages: e93669-e93669
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Role of SCOX in determination of Drosophila melanogaster lifespan.2014
Author(s)
Nguyen, T. B., Ida, H., Shimamura, M., Kitazawa, D., Akao, S., Yoshida, H., Inoue, Y. H., Yamaguchi, M.
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Journal Title
American J. Cancer Res.
Volume: 4
Pages: 325-336
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.2014
Author(s)
Yanai, H., Yoshioka, Y., Yoshida, H., Nakao, Y., Plessis, A. and Yamaguchi, M.
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Journal Title
Oncogenesis
Volume: 3
Issue: 4
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] DNA polymerase alpha interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila.2014
Author(s)
Sahashi, R., Crevel G., Pasko, J., Suyari, O., Nagai, R., Saura, M. M., Yamaguchi, M. and Cotterill, S.
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Journal Title
J. Cell Sci.
Volume: 127
Pages: 3066-3078
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] <i>Drosophila</i> DOCK Family Protein Sponge Regulates the JNK Pathway during Thorax Development2014
Author(s)
Morishita, K., Ozasa, F., Eguchi, K., Yoshioka, Y., Yoshida, H., Hiai, H. and Yamaguchi, M.
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Journal Title
Cell Structure and Function
Volume: 39
Issue: 2
Pages: 113-124
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] A genome-wide screen for mRNAs targeting the ER in SRP-independent manner, and analysis on subcellular localization of yki mRNA2014
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tesuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Hideki Yoshida
Organizer
第37回 日本分子生物学会年会
Place of Presentation
パシフィコ横浜(横浜)
Year and Date
2014-11-25 – 2014-11-27
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[Presentation] Identification of non-canonical mRNAs targeting to the ER and analysis on subcellular localization of mRNAs by RNA imaging system2014
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tesuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Henry M. Krause, Hideki Yoshida
Organizer
The Nineteenth Annual Meeting of the RNA Society
Place of Presentation
Quebec city convention centre
Year and Date
2014-06-03 – 2014-06-08
Related Report
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