| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To understand the molecular mechanism of kinetochore assembly, we tried to identify centromere-specific histone modifications and examined their significance on the centromere function. We used ChIP-seq analysis to examine centromere-specific histone modifications at chicken centromeres, which lack highly repetitive sequences. We found that H4K20 monomethylation (H4K20me1) is enriched at centromeres. We conclude that H4K20me1 modification of CENP-A nucleosomes contributes to functional kinetochore assembly. We also found that H4K5 and H4K12 acetylation (H4K5ac, H4K12ac) primarily occur within the pre-nucleosomal CENP-A-H4 complex, before centromere deposition. Based on the genetic and biochemical analyses, we conclude that H4K5ac and H4K12ac facilitate efficient CENP-A deposition into centromeres.
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