Mechanism of histone modification, which is essential for the centromere formation and contributes to maintenance of chromosome stability
| Project/Area Number |
26440190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Osaka University (2015-2016)
National Institute of Genetics (2014) |
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Principal Investigator |
HORI Tetsuya 大阪大学, 生命機能研究科, 准教授 (70550078)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | セントロメア / ヒストン修飾 / エピジェネティックス / エピジェネティクス |
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| Outline of Final Research Achievements |
To understand the molecular mechanism of kinetochore assembly, we tried to identify centromere-specific histone modifications and examined their significance on the centromere function. We used ChIP-seq analysis to examine centromere-specific histone modifications at chicken centromeres, which lack highly repetitive sequences. We found that H4K20 monomethylation (H4K20me1) is enriched at centromeres. We conclude that H4K20me1 modification of CENP-A nucleosomes contributes to functional kinetochore assembly. We also found that H4K5 and H4K12 acetylation (H4K5ac, H4K12ac) primarily occur within the pre-nucleosomal CENP-A-H4 complex, before centromere deposition. Based on the genetic and biochemical analyses, we conclude that H4K5ac and H4K12ac facilitate efficient CENP-A deposition into centromeres.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres2016
Author(s)
Shang, W. H. Hori, T. Westhorpe, F. G. Godek, K. M. Toyoda, A. Misu, S. Monma, N. Ikeo, K. Carroll, C. W. Takami, Y. Fujiyama, A. Kimura, H. Straight, A. F. Fukagawa, T.
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Journal Title
Nature communications
Volume: 7
Issue: 1
Pages: 13465-13465
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Histone H4 Lys 20 monomethylation of the CENP-A nucleosome is essential for kinetochore assembly.2014
Author(s)
Hori, T., Shang, W.H., Toyoda, A., Misu, S., Monma, N., Ikeo, K., Molina, O., Vargiu, G., Fujiyama, A., Kimura, H., Earnshaw, W.C., Fukagawa, T.
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Journal Title
Dev Cell
Volume: 29
Issue: 6
Pages: 740-749
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The CENP-O complex requirement varies among different cell types.2014
Author(s)
Kagawa, N., Hori, T., Hoki, Y., Hosoya, O., Tsutsui, K., Saga, Y., Sado, T., and *Fukagawa, T.
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Journal Title
Chrom. Research
Volume: in press
Issue: 3
Pages: 293-303
DOI
Related Report
Peer Reviewed / Open Access
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