| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In the evolutionary biology, it is very important issue to identify genes and mutations that are responsible for the adaptation. We performed a thermal adaptation experiment using the single-stranded RNA bacteriophage Qβ in which the culture temperature was increased from 37.2℃ to an inhibitory temperature of 43.6℃ in a stepwise manner in three independent lines. And we obtained three kinds of mutants that were able to amplify at 43.6℃. In this study, we measured their fitness and infection cycle quantitatively, and identified the responsible genes and substitutions for thermal adaptation of Qβ.
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