| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The evolutionary dynamics of bacterial transferred genes, which was found from the genome of Acyrthosiphon pisum, was examined. Degenerate primers were designed from LdcA1 gene originated from A. pisum and its orthologous genes, and the degenerate PCR was performed by using the genomic DNAs of 90 aphid species as templates, and the PCR products were all sequenced. From 24 species of aphids Aphidini and Macrosiphini, LdcA1 genes, which were highly homologous to A. pisum LdcA1 gene were found. The phylogenetic analysis of the deduced amino acid sequences of LdcA1 genes acquired from these 24 aphid species showed that the phylogenetic relationship of LdcA1 genes roughly reflected the present taxonomic status of these aphid species, suggesting that the LdcA1 genes were horizontally transferred to a common ancestor of these aphid groups and evolved together with speciation of these aphids groups.
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