Real-time RNAi analysis of morphological genes in the GFP-expressing water flea, Daphnia magna.

• Project/Area Number: 26440199
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Shiga Yasuhiro 東京薬科大学, 生命科学部, 助教 (00277253)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 形態進化 / ミジンコ / GFP / RNA干渉 / リアルタイム / 形態形成遺伝子 / 機能解析 / 形質転換系

Outline of Final Research Achievements

How morphological diversity of arthropods has been established is one of key questions in evolutionary developmental biology. To understand the molecular function of morphological genes during embryogenesis in the water flea, Daphnia magna, real-time RNAi analyses in a GFP-expressing strain were performed. Several time-lapse movies of developing D. magna embryos were also created.

Research Products

• [Journal Article] Analysis of spatiotemporal expression and function of the single-minded homolog in the branchiopod crustacean Daphnia magna. (2015)
  • Author(s): Morita, S., Shiga, Y., Tokishita, S., and Ohta,
  • Journal Title: Gene Volume: 555 Issue: 2 Pages: 335-345
  • DOI: 10.1016/j.gene.2014.11.028
  • Peer Reviewed
• [Journal Article] Common Transcriptional Mechanisms for Visual Photoreceptor Cell Differentiation among Pancrustaceans. (2014)
  • Author(s): Mahato, S., Morita, S., Tucker, AE, Liang, X., Jackowska, M., Friedrich, M., Shiga, Y., and Zelhof, AC.
  • Journal Title: PLoS Genetics Volume: 10 Issue: 7 Pages: e1004484-e1004484
  • DOI: 10.1371/journal.pgen.1004484
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Comprehensive study on Hox genes in the water flea, Daphnia magna (2016)
  • Author(s): Yasuhiro Shiga
  • Organizer: The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄県那覇市
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] オオミジンコ single-minded ホモログを介した正中線細胞の機能 (2016)
  • Author(s): 森田 慎一、時下 進一、志賀 靖弘、太田 敏博
  • Organizer: 第52回 日本節足動物発生学会
  • Place of Presentation: 神奈川県横須賀市
  • Year and Date: 2016-06-10
• [Presentation] オオミジンコにおける Hox 遺伝子 Antennapedia の RNAi 法による機能解析 (2014)
  • Author(s): 後藤翔平、大見川沙織、森田慎一、時下進一、志賀靖弘
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-27
• [Presentation] オオミジンコ single-minded を介した正中線細胞の機能解析 (2014)
  • Author(s): 森田慎一、志賀靖弘、時下進一、太田敏博
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-13
• [Presentation] Single-minded gene is required for specification of ventral cell by determining on ventral midline cell fate in Daphnia magna (2014)
  • Author(s): Shinichi Morita, Chisato Hiraga, Yasuhiro Shiga, Shinichi Tokishita, Taisen Iguchi, Toshihiro Ohta
  • Organizer: 第47回日本発生生物学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-28 – 2014-05-29