| Project/Area Number |
26440200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
四方 哲也 大阪大学, 情報科学研究科, 教授 (00222399)
渡邊 総一郎 東邦大学, 理学部, 准教授 (10287550)
津留 三良 大阪大学, 情報科学研究科, 助教 (80594506)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 実験室進化 / 高温適応進化 / 相互作用 / 大腸菌 / 進化 |
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| Outline of Final Research Achievements |
On the early process of E. coli 45℃ adaptive evolution, we have observed emergence and disappearance of interaction dependent growth. In this study, we first isolated E. coli clones with mutations correspond to thermo-adaptive evolution, and analyzed metabolic features (interaction dependence of growth, oxygen consumption, glucose consumption, and lactate production) of every mutant at 45℃. The results indicated adaptation pathway during 45℃ as following; 1st: alteration of mode of respiration, 2nd: accumulation of metabolites in growth media, 3rd: emergence of mutant with ability of utilization of metabolites as interactor, 4th: stabilization of intracellular environment with chaperonin mutation, 5th: recovery of mode of aspiration, and finally disappearance of interaction.
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