| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
I aim to know variations of the transferring pathways of the nutrition from the chalaza to the embryo through endosperm in the ovules of Leguminosae. Generally, the pathway was considered as follows; chalaza - endosperm - suspensor - embryo. However, the suspensor is lost by programmed cell death (PCD) at the early stage of the seed development. Therefore, I consider that the adnation between the two organs, i.e., embryo and endosperm, may be needed after the PCD. In order to know the adnating portion in legume ovules, I observed the ovules of 10 representative legume species (Gladitsia japonica, Caesalpinia decapetala, Albizia julibrissin, Cladrastis platycarpa, Maackia tashiroi, Euchresta japonica, Indigofera pseudotinctoria, Sesbania exaltata, and Lotus corniculatus) using a fluorescence microscope. I aim to detect the adnating portion by observing the loss of cuticular layer in the embryo. The layer was detected by the observation using the fluorescence microscope.
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