Environmental DNA survey for endemic and exotic giant salamanders by cooperation with citizen scientists
| Project/Area Number |
26440238
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ecology/Environment
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| Research Institution | Kobe University |
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Principal Investigator |
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| Research Collaborator |
TOMITA Sei
HIDAKA Shunsuke
FUKUMOTO Sou
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 環境DNA / リアルタイムPCR / オオサンショウウオ / 河川 / 市民科学 / チュウゴクオオサンショウウオ / 保全 / 環境教育 / 科学教育 / 保全生態学 |
|---|
| Outline of Final Research Achievements |
Detection method of environment DNA (eDNA) of Japanese giant salamander (Andrias japonicus), a special natural treasure of Japan, was established, and a detection test was conducted in rivers in Western Japan. As a result of investigating at 331 sites in the 12 rivers, eDNA of Japanese giant salamander was detected at 111 sites. Based on this result, we created an ecological niche model. The model almost matched the known distribution of the species, showing the possibility of niche modeling for rare species based on environmental DNA analysis. In the process of research, we collaborated with civic groups and high school students, and it became clear that meaningful collaboration is possible for each other.
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Environmental DNA analysis for estimating the abundance and biomass of stream fish2017
Author(s)
Doi, H., Inui, R., Akamatsu, Y., Kanno, K., Yamanaka, H., Takahara, T., Minamoto, T.
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Journal Title
Freshwater Biology
Volume: 62
Issue: 1
Pages: 30-39
DOI
Related Report
Peer Reviewed
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[Journal Article] Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio2017
Author(s)
Minamoto, T., Uchii, K., Takahara, T., Kitayoshi, T., Tsuji, S., Yamanaka, H., Doi, H.
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Journal Title
Molecular Ecology Resources
Volume: 17
Issue: 2
Pages: 324-333
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant2017
Author(s)
Yamanaka, H., Minamoto, T., Matsuura, J., Sakurai, S., Tsui, S., Motozawa, H., Hongo, M., Sogo, Y., Kakimi, N., Teramura, I., Sugita, M., Baba, M., Kondo, A.
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Journal Title
Limnology
Volume: 17
Issue: 2
Pages: 233-241
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation2016
Author(s)
Yamanaka, H., Motozawa, H., Tsuji, S., Miyazawa, R. C., Takahara, T., Minamoto, T.
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Journal Title
Ecological Research
Volume: 31
Issue: 6
Pages: 963-967
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Preliminary experiment for detection method of fish inhabiting agricultural drainage canals using environmental DNA2015
Author(s)
Koizumi, N., Takahara, T., Minamoto, T., Doi, H., Mori, A., Watanabe, K. and Takemura, T.
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Journal Title
Irrigation, Drainage and Rural Engineering Journal
Volume: 297
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Marine fish surveys using environmental DNA2014
Author(s)
Minamoto, T., Masuda, R., Takahashi, K., Maruyama, A., Yamanaka, H., Kasai, A., Kondoh, M.
Organizer
2014 ESA Annual Meeting
Place of Presentation
Sacramento, California, USA
Year and Date
2014-08-10 – 2014-08-15
Related Report
