Characterization and utilization of enlarged starch grain of rice

• Project/Area Number: 26450004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Okayama University
• Principal Investigator: Matsushima Ryo 岡山大学, 資源植物科学研究所, 准教授 (80403476)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 澱粉粒 / イネ / 胚乳 / 突然変異体 / シミュレーション / オルガネラ / アミロプラスト / 澱粉

Outline of Final Research Achievements

Starch is a biologically and commercially important polymer of glucose and is synthesized to form starch grains (SGs). Despite the simple composition of glucose polymer, SG exhibits various morphologies and sizes depending on plant species. However, the molecular mechanisms underlying this SG diversity remain unknown. To answer this question, this project analyzed rice mutants (ssg4 and ssg6), that developed enlarged SG in endosperm.

Research Products

• [Journal Article] Amyloplast Membrane Protein SUBSTANDARD STARCH GRAIN6 Controls Starch Grain Size in Rice Endosperm (2016)
  • Author(s): Matsushima, R.*, Maekawa, M., Kusano, M., Tomita, K., Kondo, H., Nishimura, H., Crofts N., Fujita N., Sakamoto W.
  • Journal Title: Plant Physiology Volume: 170 Pages: 1445-1459
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Deficiency of Starch Synthase IIIa and IVb Alters Starch Granule Morphology from Polyhedral to Spherical in Rice Endosperm (2016)
  • Author(s): Toyosawa, Y., Kawagoe, Y., Matsushima, R., Crofts, N., Ogawa, M., Fukuda, M., Kumamaru, T., Okazaki, Y., Kusano, M., Saito, K., Toyooka, K., Sato, M., Ai, Y., Jane, J.-L., Nakamura, Y., Fujita, N.
  • Journal Title: Plant Physiology Volume: 170 Pages: 1225-1270
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Geometrical formation of compound starch grains in rice implements Voronoi diagram (2015)
  • Author(s): Matsushima, R.*, Maekawa M., Sakamoto, W.
  • Journal Title: Plant Cell Physiology Volume: 56 Pages: 2150-2157
  • DOI: 10.1093/pcp/pcv123
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes. (2015)
  • Author(s): Crofts, N., Abe, N., Oitome, N. F., Matsushima, R., Hayashi, M., Tetlow, I. J., Emes, M. J., Nakamura, Y., Fujita, N.
  • Journal Title: Journal of Experimental Botany Volume: 66 Issue: 15 Pages: 4469-4482
  • DOI: 10.1093/jxb/erv212
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A mutation in GIANT CHLOROPLAST encoding a PARC6 homologue affects spikelet fertility in rice (2015)
  • Author(s): Kamau, P., Sano, S., Takami, T., Matsushima, R., Maekawa, M., and Sakamoto, W.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 5 Pages: 977-991
  • DOI: 10.1093/pcp/pcv024
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Morphological variations of starch grains. (2015)
  • Author(s): Matsushima, R.
  • Journal Title: Starch: Metabolism and Structure, Springer Volume: in press
• [Journal Article] Deficiencies in both starch synthase IIIa and branching enzyme IIb lead to a significant increase in amylose in SSIIa- inactive japonica rice seeds. (2014)
  • Author(s): Asai, H., Abe, N, Matsushima, R., Crofts, N , Oitome, N. F. , Nakamura, Y. Fujita, N.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 18 Pages: 5497-5507
  • DOI: 10.1093/jxb/eru310
  • Peer Reviewed
• [Presentation] 蛍光タンパク質によるイネとオオムギの澱粉粒の可視化 (2017)
  • Author(s): 松島 良、久野 裕
  • Organizer: 第131回日本育種学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 澱粉粒が巨大化するイネ突然変異体ssg6の解析 (2016)
  • Author(s): 松島良, 前川雅彦, 草野都, 冨田桂, 近藤秀樹, 西村秀希, クロフツ尚子, 藤田直子, 坂本亘
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学金沢八景キャンパス
  • Year and Date: 2016-03-21
• [Presentation] 穀類の澱粉粒の形状多様性についての研究 (2016)
  • Author(s): 松島 良
  • Organizer: 第8回中国地域育種談話会・第11回ムギ類研究会(共催)
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] 野生オオムギと栽培オオムギにおける胚乳細胞壁の厚さに関する解析 (2016)
  • Author(s): 最相大輔、松島 良、本庄三恵、八杉公基、永野 惇、高萩航太郎、持田恵一、武田 真、坂本 亘
  • Organizer: 第130回日本育種学会年会
  • Place of Presentation: 鳥取大学
• [Presentation] 植物が澱粉粒の大きさを決定するしくみとその利用 (2015)
  • Author(s): 松島良
  • Organizer: 粉体工学会春期研究発表会
  • Place of Presentation: 東京都千代田区 連合会館
  • Year and Date: 2015-05-18
• [Presentation] イネ胚乳の澱粉粒形成機構のシミュレーション解析 (2015)
  • Author(s): 松島良，前川雅彦，坂本亘
  • Organizer: 日本育種学会 第127回シンポジウム
  • Place of Presentation: 玉川大学
  • Year and Date: 2015-03-22
• [Presentation] 穀類の澱粉粒の形状多様性についての研究 (2014)
  • Author(s): 松島良
  • Organizer: おかやまバイオアクティフ研究会 第45回シンポジウム
  • Place of Presentation: 倉敷市芸文館 アイシアター
  • Year and Date: 2014-06-27
  • Invited