An investigation of the cause of seed enlargement due to the loss of IRE1
Project/Area Number |
26450010
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Science in genetics and breeding
|
Research Institution | Osaka Prefecture University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 小胞体ストレス応答 / シロイヌナズナ / 種子 / IRE1 / 遺伝子導入 |
Outline of Final Research Achievements |
The present study shows that the enlargement of seeds of Arabidopsis ire1 mutant (ire1a and ire1b double mutant) is caused by an increase the amount of protein bodies. Furthermore, we introduced wild type or mutated FLAG-tagged IRE1A/B genes into ire1 mutant and analyzed the transgenic plants. From these results, activation of IRE1 without sensor domain may be involved in the seed size mutation. This unusual IRE1 activation may be due to a change in the fatty acid composition of the ER membrane, unlike the ER stress response.
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Report
(4 results)
Research Products
(10 results)