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An investigation of the cause of seed enlargement due to the loss of IRE1

Research Project

Project/Area Number 26450010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionOsaka Prefecture University

Principal Investigator

Mishiba Kei-ichiro  大阪府立大学, 生命環境科学研究科, 准教授 (70390888)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords小胞体ストレス応答 / シロイヌナズナ / 種子 / IRE1 / 遺伝子導入
Outline of Final Research Achievements

The present study shows that the enlargement of seeds of Arabidopsis ire1 mutant (ire1a and ire1b double mutant) is caused by an increase the amount of protein bodies. Furthermore, we introduced wild type or mutated FLAG-tagged IRE1A/B genes into ire1 mutant and analyzed the transgenic plants. From these results, activation of IRE1 without sensor domain may be involved in the seed size mutation. This unusual IRE1 activation may be due to a change in the fatty acid composition of the ER membrane, unlike the ER stress response.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015 2014 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] Activation of the Arabidopsis membrane-bound transcription factor bZIP28 is mediated by site-2 protease, but not site-1 protease2017

    • Author(s)
      Yuji Iwata, Makoto Ashida, Chisa Hasegawa, Kazuki Tabara, Kei-ichiro Mishiba, Nozomu Koizumi
    • Journal Title

      The Plant Journal

      Volume: 印刷中 Issue: 3 Pages: 408-415

    • DOI

      10.1111/tpj.13572

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis tRNA ligase completes the cytoplasmic splicing of bZIP60 mRNA in the unfolded protein response.2016

    • Author(s)
      Yukihiro Nagashima, Yuji Iwata, Kei-ichiro Mishiba, Nozomu Koizumi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 470 Issue: 4 Pages: 941-946

    • DOI

      10.1016/j.bbrc.2016.01.145

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tunicamycin-induced inhibition of protein secretion into culture medium of Arabidopsis T87 suspension cells through mRNA degradation on the endoplasmic reticulum2016

    • Author(s)
      Yuji Iwata, Noriko Hayashi, Kazuki Tabara, Kei-ichiro Mishiba, Nozomu Koizumi
    • Journal Title

      Bioscience, biotechnology, and biochemistry

      Volume: 印刷中 Issue: 6 Pages: 1168-1171

    • DOI

      10.1080/09168451.2016.1151340

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exogenous salicylic acid activates two signaling arms of the unfolded protein response in Arabidopsis.2014

    • Author(s)
      Yukihiro Nagashima, Yuji Iwata, Makoto Ashida, Kei-ichiro Mishiba and Nozomu Koizumi.
    • Journal Title

      Plant & Cell Physiology

      Volume: 55 Issue: 10 Pages: 1772-1778

    • DOI

      10.1093/pcp/pcu108

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 細胞質スプライシングをモニターする形質転換シロイヌナズナの作出と解析2015

    • Author(s)
      田原一喜、三柴啓一郎、小泉望、岩田雄二
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2015-08-10
    • Related Report
      2015 Research-status Report
  • [Presentation] Ligation of bZIP60 mRNA cleaved by IRE1 in Arabidopsis2015

    • Author(s)
      Yukihiro Nagashima, Yuji Iwata, Kei-ichiro Mishiba and Nozomu Koizumi
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular mechanism of proteolytic activation of bZIP28, an Arabidopsis membrane-bound transcription factor involved in the unfolded protein response2015

    • Author(s)
      Makao Ashida, Yuji Iwata, Kei-ichiro Mishiba and Nozomu Koizumi
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] IRE1による細胞質スプライシングの生化学的解析2014

    • Author(s)
      長島幸広、岩田雄二、三柴啓一郎、小泉望
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      いわて県民情報交流センター
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Research-status Report
  • [Remarks] 植物分子育種学ホームページ

    • URL

      http://www.biosci.osakafu-u.ac.jp/pmb/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究グループHP

    • URL

      http://www.plant.osakafu-u.ac.jp/~mishiba/

    • Related Report
      2015 Research-status Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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