| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In Japan, cultivated radishes show huge diversity that can not be observed in other countries. In order to know genetic roles of the wild radishes to the development of diversity in Japanese cultivars, differentiations of a mitochondrial cytoplasmic male sterile gene (orf138) and fertility restoration genes (Rf genes ) in nucleus were observed. Also, the relationships between the presence of Rf genes and the actual pollen fertility were investigated. It was found by PCR of orf138 gene that most of the modern radish varieties possess orf138, the fact indicating that male sterile gene has been rapidly introduced into cultivars. A half of the investigated varieties possessed Rf genes. By the observation of phenotypic pollen fertility, it was found that several varieties demonstrated male sterility despite the fact that they have the Rf gene. Complex relationship between orf138 and Rf genes were estimated.
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