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Development of DNA marker associated with virus resistance for papaya breeding.

Research Project

Project/Area Number 26450014
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionOkinawa Prefectural Agricultural Research Center

Principal Investigator

Urasaki Naoya  沖縄県農業研究センター, 本所研究企画班, 班長 (20504591)

Co-Investigator(Kenkyū-buntansha) 太郎良 和彦  沖縄県農業研究センター, 本所研究企画班, 上席主任研究員 (10504772)
Co-Investigator(Renkei-kenkyūsha) MATSUMURA Hideo  信州大学, 基盤研究支援センター・生命科学分野・遺伝子実験支援部門, 准教授 (40390885)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsウイルス / 抵抗性 / 遺伝子 / 属間交雑 / ゲノム育種・マーカー育種 / 熱帯果樹 / パパイヤ / ウイルス抵抗性
Outline of Final Research Achievements

To breed virus resistant papaya, intergeneric hybrids between papaya and mountain papaya, and backcross plants between intergeneric hybrids and papaya were developed. Both intergeneric hybrids and backcross plants showed resistance to papaya leaf distortion mosaic virus. According to the RAD-seq analysis of intergeneric hybrids and the previous reports, we identified a STK gene as candidate of virus resistance gene and converted it into a CAPS marker which can be usable for papaya breeding.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Development of plants resistant to <i>Papaya leaf distortion mosaic virus</i> by intergeneric hybridization between <i>Carica papaya</i> and <i>Vasconcellea cundinamarcensis</i>2016

    • Author(s)
      Tarora, K., Shudo, A., Kawano, S., Yasuda, K., Ueno, H., Matsumura, H. and Urasaki, N.
    • Journal Title

      Breeding Science

      Volume: 66 Issue: 5 Pages: 734-741

    • DOI

      10.1270/jsbbs.16107

    • NAID

      130005290474

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 属間雑種(パパイヤ×マウンテンパパイヤ)の戻し交雑個体におけるパパイヤ奇形葉モザイクウイルス抵抗性評価2017

    • Author(s)
      太郎良和彦・河野伸二・安田慶次・玉城盛俊・松村英生・浦崎直也
    • Organizer
      日本育種学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 属間雑種(パパイヤ×マウンテンパパイヤ)の戻し交雑個体における性型とゲノムサイズの変化2016

    • Author(s)
      太郎良和彦、安田慶次、河野伸二、上野広樹、松村英生、浦崎直也
    • Organizer
      日本育種学会 第129回講演会
    • Place of Presentation
      横浜市立大学
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] マウンテンパパイヤが持つパパイヤ奇形葉モザイクウイルス抵抗性遺伝子の探索2015

    • Author(s)
      太郎良和彦、屋比久裕也、兼島莉沙、河野伸二、安田慶次、上野広樹、松村英生、浦崎直也
    • Organizer
      日本育種学会 第128回講演会
    • Place of Presentation
      新潟大学
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] パパイアとマウンテンパパイアの属間交雑へのパパイアの戻し交雑2015

    • Author(s)
      太郎良 和彦,屋比久 裕也,宮里 千春,高嶺 朝典,安田 慶次,河野 伸二,上野 広樹,松村 英生,浦崎 直也
    • Organizer
      日本育種学会 第127回講演会
    • Place of Presentation
      玉川大学
    • Year and Date
      2015-03-22
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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