| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We have identified a ryegrass brittle culm 1 gene (LmBC1) for cellulose biosynthesis on a genetic map of a linkage group (LG) 4 of Italian ryegrass by conducting DNA marker analysis with a BC1F1 population derived from backcrossing a F1 progeny to a mutant individual that showed qualitative and recessive phenotype of culm brittleness―the F1 progeny was derived from a single cross between a wild-type individual and the mutant individual. The order of the rice gene-derived markers mapped around the LmBC1 on the genetic map was well correlated with the physical order of the corresponding genes in rice chromosome (Chr) 3 that is syntenic to the LG 4. Data analysis with public database of the rice gene expression profile revealed that the rice brittle culm 1 gene (BC1) locates on the rice Chr 3, and both the LmBC1 and BC1 locate between the same markers/genes in the LG 4 and rice Chr 3, respectively. These results strongly indicate that the LmBC1 is an ortholog of the BC1.
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