Identification of a genetic locus and dynamic analysis of genes for cellulose biosynthesis in ryegrasses
Project/Area Number |
26450026
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Crop production science
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
TAKAHASHI Wataru 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門飼料作物研究領域, 上級研究員 (70455319)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | イネ科牧草 / カマイラズ / 消化性 / 植物二次細胞壁 / セルロース合成変異 / バイオマス / ライグラス |
Outline of Final Research Achievements |
We have identified a ryegrass brittle culm 1 gene (LmBC1) for cellulose biosynthesis on a genetic map of a linkage group (LG) 4 of Italian ryegrass by conducting DNA marker analysis with a BC1F1 population derived from backcrossing a F1 progeny to a mutant individual that showed qualitative and recessive phenotype of culm brittleness―the F1 progeny was derived from a single cross between a wild-type individual and the mutant individual. The order of the rice gene-derived markers mapped around the LmBC1 on the genetic map was well correlated with the physical order of the corresponding genes in rice chromosome (Chr) 3 that is syntenic to the LG 4. Data analysis with public database of the rice gene expression profile revealed that the rice brittle culm 1 gene (BC1) locates on the rice Chr 3, and both the LmBC1 and BC1 locate between the same markers/genes in the LG 4 and rice Chr 3, respectively. These results strongly indicate that the LmBC1 is an ortholog of the BC1.
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Report
(4 results)
Research Products
(4 results)