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Breeding for Japanese pear having low-chillin requirement and 1dentification of major genes related to endodormancy

Research Project

Project/Area Number 26450034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionTottori University

Principal Investigator

Tamura Fumio  鳥取大学, 農学部, 教授 (50217197)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords温暖化適応 / ナシ品種 / 休眠機構 / 低温要求量 / 自家和合性 / 温暖化 / 休眠打破 / 遺伝子発現 / ナシ / 育種 / 適応 / 自発休眠 / QTL解析 / 遺伝 / ニホンナシ
Outline of Final Research Achievements

We investigated chilling requirement (CR) in Taiwanese pear Yokoyama and Japanese pear TH3 (S1 of ‘Osa-Nijisseiki’) and their F1 of No. 72, and its F2. CR in F2 plants was widely distributed between that of Yokoyama and TH3. From these results, it was suggested that pear plants had quantitative trait loci determining CR as a genetic factor. A hundred twenty DNA polymorphisms containing NAC-domain protein and F-box protein were detected by Restriction-site Associated DNA Sequencing between the F2 plants having low and high CR. These results indicated that chilling CR in pear plants was controlled by the plant hormone receptor activity.
Some progenies from Akibae and No.72 have low chilling requirement raged 300 to 600 CU, self-compatibility and well fruit quality.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (7 results)

  • [Journal Article] Pear pollen selection using higher germination properties at low temperatures and the effect on the fruit set and quality of Japanese pear cultivars2017

    • Author(s)
      K. Kuroki, Y. Takemura, M. Jiang, H. Marumori, N. Teratani, K. Matsumoto, T. Matsumoto and F. Tamura
    • Journal Title

      Scientia Horticulturae

      Volume: 216 Pages: 200-204

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Identification of the expressed protein and the impact of change in ascorbate peroxidase activity related to endodormancy breaking in Pyrus pyrifolia2015

    • Author(s)
      Y. Takemura, K. Kuroki, M. Jiang, K. Matsumoto, F. Tamura :
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 86 Pages: 121-121

    • DOI

      10.1016/j.plaphy.2015.08.017

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparative transcriptome analysis of the less-dormant Taiwanese pear and the dormant Japanese pear during winter season.2015

    • Author(s)
      Y. Takemura, K. Kuroki, Y. Shida, S. Araki, Y. Takeuchi, K. Tanaka, T. Ishige, S. Yajima, F. Tamura
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 10 Pages: e0139595-e0139595

    • DOI

      10.1371/journal.pone.0139595

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of the expressed protein and the impact of change in ascorbate peroxidase activity related to endodormancy breaking in Pyrus pyrifolia2015

    • Author(s)
      Yoshihiro Takemura, Katsuou Kuroki, Mingfeng Jiang, Kazuhiro Matsumoto, Fumio Tamura
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 86 Pages: 121-129

    • DOI

      10.1016/j.plaphy.2014.11.016

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] RAD-seq analysis of genes related to chilling requirements for endodormancy breaking in pyrus plant.2017

    • Author(s)
      Y. Takemura, Y. Shida, K. Kuroki, K. Tanaka, S. Yajima F. Tamura
    • Organizer
      4th International Symposium on Molecular Markers in Horticulture
    • Related Report
      2017 Annual Research Report
  • [Presentation] ニホンナシの自発休眠打破と耐凍性に及ぼすABAおよびシアナミド処理の影響2017

    • Author(s)
      稲本俊彦・宮川紗綾・坂廼辺忠幸・竹村圭弘・田村文男
    • Organizer
      園芸学会平成29年度秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナシの低温要求性に関与する遺伝子の RAD-Seq 解析2016

    • Author(s)
      竹村圭弘 ・志田曜司 ・稲本俊彦 ・荒木駿吾 ・竹内ゆかり ・黒木克翁 ・田村文男
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学(名古屋市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] :次世代シーケンサーを用いたトランスクリプトーム解析によるナシの芽の自発休眠打破機構の解明2015

    • Author(s)
      竹内ゆかり・竹村圭弘・志田曜司・荒木駿吾・黒木克翁・蒋明凰・田村文男
    • Organizer
      園芸学会
    • Place of Presentation
      徳島市
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] ニホンナシ系統TH3とタイワンナシ横山とのF1およびF2系統群における自発休眠導入特性2015

    • Author(s)
      荒木駿吾・竹村圭弘・志田曜司・竹内ゆかり・黒木克翁・蒋明鳳・田村文男
    • Organizer
      園芸学会
    • Place of Presentation
      徳島市
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 次世代シーケンサーを用いたトランスクリプトーム解析によるナシの芽の自発休眠打破機構の解明2014

    • Author(s)
      竹村圭弘・志田曜司・荒木駿吾・竹内ゆかり・黒木克翁・蒋明3・田村文男
    • Organizer
      園芸学会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Research-status Report
  • [Presentation] ニホンナシ系統TH3とタイワンナシ横山とのF1およびF2系統群における自発休眠導入特性2014

    • Author(s)
      志田曜司・竹村圭弘・荒木駿吾・竹内ゆかり・黒木克翁・蒋明鳳・田村文男
    • Organizer
      園芸学会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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