| Project/Area Number |
26450050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant protection science
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| Research Institution | Rakuno Gakuen University (2016-2017)
The University of Tokyo (2014-2015) |
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Principal Investigator |
Komoda Yuka 酪農学園大学, 農食環境学群, 講師 (90716482)
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| Co-Investigator(Kenkyū-buntansha) |
中原 健二 北海道大学, 農学研究院, 講師 (90315606)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ポティウイルス / 遺伝子発現 / フレームシフト / 培養細胞 / マメ科 / 核果類 / プロトプラスト / 遺伝子導入 |
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| Outline of Final Research Achievements |
Potyviruses cause diseases in many important crops. To reveal the replication mechanism of potyviruses, we attempted to generate an in vitro potyviral translation-replication system using their natural host plants. The establishment of the natural host-derived in vitro system remains incomplete. We investigated the expression mechanism of pipo ORF discovered recently in potyviral genomes, by using conventional in vitro and in vivo assay systems. The pipo ORF exists in the -1 (or +2) reading frame relative to the viral polyprotein ORF, and is expressed as a fusion protein with the N-terminal half of P3 (P3N-PIPO). We revealed that the pipo ORF is expressed via transcriptional slippage during viral replication. We also found that, in addition to P3N-PIPO, another frameshift product, P3N-ALT, is also produced via transcriptional slippage.
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