Transgenic stacking to improve plant disease resistance against mycotoxin-producing fungi.

• Project/Area Number: 26450053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kanazawa University
• Principal Investigator: Nishiuchi Takumi 金沢大学, 学際科学実験センター, 准教授 (20334790)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 病害抵抗性 / トリコテセン系かび毒 / マイコトキシン / 赤かび病 / スタッキング / 解毒 / 植物病原糸状菌 / かび毒 / トリコテセン / ユビキチン

Outline of Final Research Achievements

Fusarium head blight (FHB) is serious disease leading to significant yield loss and accumulation of trichothecene mycotoxins. To improve disease resistance and mycotoxin detoxification in plants against F. graminearum, we generated transgenic stacking in Arabidopsis plants. We had identified some defense-related genes that are involved in the disease resistance against F. graminearum. Among these genes, we revealed that the ubq6/6* double mutant strong disease resistance. The barley HvGST13 and HvGR2 genes were specifically upregulated in the barley breeding lines showing lower mycotoxin (DON) accumulation. The HvGST13ox and HvGR2ox Arabidopsis plants were DON-resistant phenotypes compared to wild type. In addition, HvGST13ox Arabidopsis plants showed weak disease symptoms and low DON accumulation after inoculation of F. graminearum conidia. In the future, crossing the ubq6/6* mutant with HvGST13ox and HvGR2ox likely shows low disease symptoms and DON accumulation by F. graminearum.

Research Products

• [Journal Article] The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis. (2017)
  • Author(s): Sayo Kodama, Junya Ishizuka, Ito Miyashita, Takaaki Ishii, Takumi Nishiuchi, Hideto Miyoshi, Yasuyuki Kubo
  • Journal Title: PLOS Pathogens Volume: - Issue: 2 Pages: e1006189-e1006189
  • DOI: 10.1371/journal.ppat.1006189
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dysfunction of Arabidopsis MACPF domain protein activates programmed cell death via tryptophan metabolism in MAMP-triggered immunity (2017)
  • Author(s): Fukunaga S, Sogame M, Hata M, Singkaravanit-Ogawa S, Pislewska-Bednarek M, Onozawa-Komori M, Nishiuchi T, Hiruma K, Saitoh H, Terauchi R, Kitakura S, Inoue Y, Bednarek P, Schulze-Lefert P, and Takano Y.
  • Journal Title: Plant Journal Volume: 89 Issue: 2 Pages: 381-393
  • DOI: 10.1111/tpj.13391
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of the acivicin effects on trichothecene production by Fusarium graminearum species complex. (2016)
  • Author(s): Maeda K, Nakajima Y, Tanahashi Y, Kosaki T, Kitou Y, Kanamaru K, Kobayashi T, Nishiuchi T, Kimura M.
  • Journal Title: J Gen Appl Microbiol. Volume: 62 Pages: 272-276
  • NAID: 130005284317
  • Peer Reviewed
• [Journal Article] Hydroxylations of trichothecene rings in the biosynthesis of Fusarium trichothecenes: evolution of alternative pathways in the nivalenol chemotype. (2016)
  • Author(s): Maeda K, Tanaka A, Sugiura R, Koshino H, Tokai T, Sato M, Nakajima Y, Tanahashi Y, Kanamaru K, Kobayashi T, Nishiuchi T, Fujimura M, Takahashi-Ando N, Kimura M.
  • Journal Title: Environ Microbiol. Volume: 18 Issue: 11 Pages: 3798-3811
  • DOI: 10.1111/1462-2920.13338
  • Peer Reviewed
• [Journal Article] Colletotrichum orbiculare WHI2, a Yeast Stress-Response Regulator Homolog, Controls the Biotrophic Stage of Hemibiotrophic Infection Through TOR Signaling (2016)
  • Author(s): Ken Harata, Takumi Nishiuchi and Yasuyuki Kubo
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 29 Issue: 6 Pages: 468-483
  • DOI: 10.1094/mpmi-02-16-0030-r
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Re-examination of genetic and nutritional factors related to trichothecene biosynthesis in Fusarium graminearum. (2016)
  • Author(s): Kitou Y, Nakajima Y, Maeda K, Jin Q, Nishiuchi T, Kanamaru K, Kobayashi T, Kimura M.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 80 Issue: 2 Pages: 414-417
  • DOI: 10.1080/09168451.2015.1088374
  • Peer Reviewed
• [Journal Article] Effect of disrupting the trichothecene efflux pump encoded by <i>FgTri12</i> in the nivalenol chemotype of <i>Fusarium graminearum</i> (2015)
  • Author(s): Nakajima, Y., Koseki, N., Sugiura, R., Tominaga, N., Maeda, K., Tokai, T., Izawa, M., Kanamaru, K., Kamakura, T., Kobayashi, T., Nishiuchi, T., Yoshida, M., and Kimura, M.
  • Journal Title: The Journal of General and Applied Microbiology Volume: 61 Issue: 3 Pages: 93-96
  • DOI: 10.2323/jgam.61.93
  • NAID: 130005090099
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed
• [Journal Article] Responses to intermittent exposure to green light during the dark period in <i>Arabidopsis thaliana</i> and possible involvement of cryptochrome 2 (2015)
  • Author(s): Sato, M., Nishiuchi, T. and Sakamoto, T.
  • Journal Title: Plant Biotechnology Volume: 32 Issue: 3 Pages: 263-266
  • DOI: 10.5511/plantbiotechnology.15.0729a
  • NAID: 130005105915
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] The mode of phytotoxic action of trichothecenes during plant-<i>Fusarium</i> interaction; our approach to detoxification of trichothecenes in cereals. (2015)
  • Author(s): 西内 巧，木村 真，佐藤 和広
  • Journal Title: JSM Mycotoxins Volume: 65 Issue: 2 Pages: 143-147
  • DOI: 10.2520/myco.65.143
  • NAID: 130005096242
  • ISSN: 0285-1466, 1881-0128
  • Peer Reviewed
• [Journal Article] Effects of acivicin on growth, mycotoxin production and virulence of phytopathogenic fungi (2014)
  • Author(s): Maeda, K., Nakajima, Y., Motoyama, T., Kitou, Y., Kosaki, T., Saito, T., Nishiuchi, T., Kanamaru, K., Osada, H., Kobayashi, T., Kimura, M.
  • Journal Title: Letters in Applied Microbiology Volume: 59 Issue: 4 Pages: 377-383
  • DOI: 10.1111/lam.12289
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 植物由来の代謝物を用いた赤かび病防除及びかび毒低減化技術の開発 (2017)
  • Author(s): 西内巧、澤田有司、平井優美、 中島祐、佐藤和広、木村真
  • Organizer: 日本農薬学会第４２回大会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2017-03-06
• [Presentation] 炭疽病菌およびいもち病菌においてRabGAP Bub2は細胞周期および隔壁形成を制御し植物感染に関与する (2016)
  • Author(s): 深田史美、西内巧、久保康之
  • Organizer: 平成28年度植物病理学会関西部会
  • Place of Presentation: 静岡県コンベンションアーツセンター
  • Year and Date: 2016-09-29
• [Presentation] ウリ類炭疽病菌におけるMOR経路因子CoPAG1は植物シグナル認識を介した遺伝子発現の制御に関与する (2016)
  • Author(s): 小玉紗代，西内巧，久保康之
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2016-03-21
• [Presentation] かび毒産生抑制技術の開発を目指したトリコテセン生合成制御機構の解析 (2016)
  • Author(s): 中嶋佑一、塩原拓也、前田一行、棚橋義和、金丸京子、小林哲夫、西内巧、木村真
  • Organizer: 日本マイコトキシン学会第 79 回学術講演会
  • Place of Presentation: 文部科学省研究交流センター
• [Presentation] ニバレノール生合成の分子遺伝学－生合成酵素および alternative hydroxylaseによるトリコテセン骨格の水酸化 (2015)
  • Author(s): 前田一行、越野広雪、田中彰、杉浦涼介、東海武史、佐藤真之、棚橋和義、中嶋佑一、 金丸京子、小林哲夫、西内 巧、藤村 真、安藤直子、木村 真
  • Organizer: 日本マイコトキシン学会第 77 回学術講演会
  • Place of Presentation: 山形県鶴岡市
  • Year and Date: 2015-09-11
• [Presentation] NAD及び関連代謝物による植物の病害抵抗性誘導の作用機構 (2015)
  • Author(s): 三輪晃敬、澤田有司、平井優美、佐藤和広、西内巧
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ニコチンアミドモノヌクレオチドによる植物の病害抵抗性誘導の解析 (2014)
  • Author(s): 三輪晃敬、澤田有司、平井優美、佐藤和広、西内巧
  • Organizer: 平成26年度植物病理学会関西部会
  • Place of Presentation: 富山大学
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] 赤かび病抵抗性に関わるUBQ/RPS27a遺伝子の機能解析 (2014)
  • Author(s): 三輪晃敬、西村浩二、佐藤雅彦、加藤智朗、古賀博則、木村真、佐藤和広、西内巧
  • Organizer: 平成26年度植物病理学会関西部会
  • Place of Presentation: 富山大学
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] ニコチンアミドモノヌクレオチドによる植物の病害抵抗性誘導の解析 (2014)
  • Author(s): 三輪晃敬、澤田有司、平井優美、佐藤和広、西内巧
  • Organizer: 平成26年度植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター(札幌市・北海道)
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] 赤かび病抵抗性及びかび毒感受性に関わるUBQ/RPS27a遺伝子の機能解析 (2014)
  • Author(s): 佐々木亮輔、三輪晃敬、加藤智朗、古賀博則、木村真、佐藤和広、西内巧
  • Organizer: 平成26年度植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター(札幌市・北海道)
  • Year and Date: 2014-06-02 – 2014-06-04
• [Book] Methods Mol Biol. (2014)
  • Author(s): Asano T,Nishiuchi T.
  • Publisher: Springer
• [Patent(Industrial Property Rights)] 赤かび病菌かび毒産生抑制剤 (2015)
  • Inventor(s): 木村真、前田一行、中嶋佑一、西内巧、三輪晃敬
  • Industrial Property Rights Holder: 木村真、前田一行、中嶋佑一、西内巧、三輪晃敬
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-226384
  • Filing Date: 2015-11-19
• [Patent(Industrial Property Rights)] 赤かび病抵抗性植物、その作製方法及びその利用 (2014)
  • Inventor(s): 西内巧、三輪晃敬、佐藤和広
  • Industrial Property Rights Holder: 西内巧、三輪晃敬、佐藤和広
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-180661
  • Filing Date: 2014-09-04