Transgenic stacking to improve plant disease resistance against mycotoxin-producing fungi.
| Project/Area Number |
26450053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant protection science
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| Research Institution | Kanazawa University |
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Principal Investigator |
Nishiuchi Takumi 金沢大学, 学際科学実験センター, 准教授 (20334790)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 病害抵抗性 / トリコテセン系かび毒 / マイコトキシン / 赤かび病 / スタッキング / 解毒 / 植物病原糸状菌 / かび毒 / トリコテセン / ユビキチン |
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| Outline of Final Research Achievements |
Fusarium head blight (FHB) is serious disease leading to significant yield loss and accumulation of trichothecene mycotoxins. To improve disease resistance and mycotoxin detoxification in plants against F. graminearum, we generated transgenic stacking in Arabidopsis plants. We had identified some defense-related genes that are involved in the disease resistance against F. graminearum. Among these genes, we revealed that the ubq6/6* double mutant strong disease resistance. The barley HvGST13 and HvGR2 genes were specifically upregulated in the barley breeding lines showing lower mycotoxin (DON) accumulation. The HvGST13ox and HvGR2ox Arabidopsis plants were DON-resistant phenotypes compared to wild type. In addition, HvGST13ox Arabidopsis plants showed weak disease symptoms and low DON accumulation after inoculation of F. graminearum conidia. In the future, crossing the ubq6/6* mutant with HvGST13ox and HvGR2ox likely shows low disease symptoms and DON accumulation by F. graminearum.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Dysfunction of Arabidopsis MACPF domain protein activates programmed cell death via tryptophan metabolism in MAMP-triggered immunity2017
Author(s)
Fukunaga S, Sogame M, Hata M, Singkaravanit-Ogawa S, Pislewska-Bednarek M, Onozawa-Komori M, Nishiuchi T, Hiruma K, Saitoh H, Terauchi R, Kitakura S, Inoue Y, Bednarek P, Schulze-Lefert P, and Takano Y.
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Journal Title
Plant Journal
Volume: 89
Issue: 2
Pages: 381-393
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Hydroxylations of trichothecene rings in the biosynthesis of Fusarium trichothecenes: evolution of alternative pathways in the nivalenol chemotype.2016
Author(s)
Maeda K, Tanaka A, Sugiura R, Koshino H, Tokai T, Sato M, Nakajima Y, Tanahashi Y, Kanamaru K, Kobayashi T, Nishiuchi T, Fujimura M, Takahashi-Ando N, Kimura M.
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Journal Title
Environ Microbiol.
Volume: 18
Issue: 11
Pages: 3798-3811
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of disrupting the trichothecene efflux pump encoded by <i>FgTri12</i> in the nivalenol chemotype of <i>Fusarium graminearum</i>2015
Author(s)
Nakajima, Y., Koseki, N., Sugiura, R., Tominaga, N., Maeda, K., Tokai, T., Izawa, M., Kanamaru, K., Kamakura, T., Kobayashi, T., Nishiuchi, T., Yoshida, M., and Kimura, M.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 61
Issue: 3
Pages: 93-96
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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[Journal Article] Effects of acivicin on growth, mycotoxin production and virulence of phytopathogenic fungi2014
Author(s)
Maeda, K., Nakajima, Y., Motoyama, T., Kitou, Y., Kosaki, T., Saito, T., Nishiuchi, T., Kanamaru, K., Osada, H., Kobayashi, T., Kimura, M.
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Journal Title
Letters in Applied Microbiology
Volume: 59
Issue: 4
Pages: 377-383
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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