Structual analysis of R protein activation

• Project/Area Number: 26450055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Yokohama City University (2016); Nara Institute of Science and Technology (2014-2015)
• Principal Investigator: Kawano Yoji 横浜市立大学, 木原生物学研究所, 客員准教授 (00406175)
• Co-Investigator(Kenkyū-buntansha): 安田 伸子 国立研究開発法人農業・食品産業技術総合研究機構, その他部局等, 研究員 (00355504) ; 河野 貴子 (金児貴子) 立命館大学, 薬学部, 准教授 (00378019)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: イネ / R タンパク質 / 抵抗性タンパク質 / 耐病性 / 進化 / 遺伝子

Outline of Final Research Achievements

Although resistant (R) proteins are important intracellular immune receptors that induce the strongest immune response in plants, it is difficult to purify full-length R proteins, so signal transduction and activation mechanisms of R proteins remain largely unclear. We have previously reported that rice R protein Pit confers resistance to rice blast fungus and plays important roles in rice innate immunity. However, it is largely unknown how Pit induces downstream immune responses. In this study, we performed the biochemical analyses using purified the CC domain and the NB-ARC domain of Pit recombinant proteins to clarify activation mechanisms of Pit-1. We found that the exchange of ADP/ATP binding in the NB-ARC domain of Pit influences the intramolecular interaction between the CC domain and the NB-ARC domain of Pit. Moreover, we demonstrated here that the CC domain of Pit forms a dimer and a super oligomer in solution.

Research Products

• [Int'l Joint Research] Ohio State University(米国)
• [Int'l Joint Research] Institute of Plant Protection/Hunan Agricultural University/Shanghai Center for Plant Stress Biology(中国)
• [Journal Article] Crosstalk of Signaling Mechanisms Involved in Host Defense and Symbiosis against Microorganisms in Rice (2016)
  • Author(s): Akamatsu, A., Shimamoto, K., and Kawano, Y.
  • Journal Title: Curr Genomic Volume: 掲載予定
  • Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice. (2015)
  • Author(s): Akamatsu, A., Uno, K., Kato, M., Wong, HL., Shimamoto, K., and Kawano, Y.
  • Journal Title: Plant Signal Behav Volume: 10
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice. (2015)
  • Author(s): Liu, L., Park, CH., He, F., Nagano, M., Wang, M., Bellizzi, M., Zeng, X., Liu, W., Ning, Y., Kawano, Y., and Wang, GL.
  • Journal Title: PLoS Pathog Volume: 11
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice. (2015)
  • Author(s): Liu, L., Park, CH., He, F., Nagano, M., Wang, M., Bellizzi, M., Zeng, X., Liu, W., Ning, Y.,Kawano, Y., and Wang, GL.
  • Journal Title: PLoS Pathog. Volume: 11 Issue: 2 Pages: e1004629-e1004629
  • DOI: 10.1371/journal.ppat.1004629
  • Peer Reviewed
• [Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase. (2015)
  • Author(s): Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 41 Pages: 28569-78
  • DOI: 10.1074/jbc.m114.603282
  • Description: (in press)(印刷中)
  • Peer Reviewed
• [Journal Article] Palmitoylation-dependent membrane localization of the rice R protein Pit is critical for the activation of the small GTPase OsRac1. (2015)
  • Author(s): Kawano, Y., Fujiwara, T., Yao, A., Housen, Y., Hayashi, K., and Shimamoto, K.
  • Journal Title: J Biol Chem. Volume: 289 Issue: 27 Pages: 19079-88
  • DOI: 10.1074/jbc.m114.569756
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rho family GTPase-dependent immunity in plants and animals. (2014)
  • Author(s): Kawano, Y., Kawano-Kaneko, T., and Shimamoto, K.
  • Journal Title: Front. Plant Sci. Volume: 5 Pages: 522-522
  • DOI: 10.3389/fpls.2014.00522
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance (2014)
  • Author(s): Cesari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., Kroj, T.
  • Journal Title: EMBO Journal Volume: 33 Issue: 17 Pages: 1941-1959
  • DOI: 10.15252/embj.201487923
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] α-Taxilin interacts with soring nexin 4 and participates in the recycling pathway of transferrin receptor. (2014)
  • Author(s): Sakane, H., Horii, Y., Nogami, S., Kawano, Y., Kaneko-Kawano, T., and Shirataki, H.
  • Journal Title: PloS ONE Volume: 9 Issue: 4 Pages: e93509-e93509
  • DOI: 10.1371/journal.pone.0093509
  • Peer Reviewed / Open Access
• [Presentation] Elucidation of mechanisms of small GTPase OsRac1 activation by R protein Pit-1 through OsSPK1 (2016)
  • Author(s): Yoji Kawano
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター (岡山県岡山市)
  • Year and Date: 2016-03-21
• [Presentation] Elucidation of mechanisms of small GTPase OsRac1 activation by R protein Pit-1 through OsSPK1 (2016)
  • Author(s): Yoji Kawano
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県盛岡)
  • Year and Date: 2016-03-18
• [Presentation] Elucidation of mechanisms of small GTPase OsRac1 activation by R protein Pit-1 through OsSPK1 (2015)
  • Author(s): Yoji Kawano
  • Organizer: The 4th International Conference on Biotic Plant Interactions (国際学会)
  • Place of Presentation: 南京 （中国）
  • Year and Date: 2015-07-31
  • Int'l Joint Research
• [Presentation] Small GTPase OsRac1 is a key regulator in PAMP-triggered immunity and effector-triggered immunity (2015)
  • Author(s): Yoji Kawano
  • Organizer: 3rd Beijing International Symposium on Molecular Plant Pathology(招待講演)(国際学会)
  • Place of Presentation: 北京 （中国）
  • Year and Date: 2015-07-28
  • Int'l Joint Research / Invited
• [Presentation] 抵抗性タンパク質Pitは、OsSPIKE1を介した低分子量Gタンパク質OsRac1活性化により耐病性を制御する (2015)
  • Author(s): 河野洋治、島本功
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都、世田谷区）
  • Year and Date: 2015-03-16
• [Remarks] PSC
  • URL: http://www.psc.ac.cn/research_personal.asp?id=144&Yoji%20Kawano
• [Remarks] Kawano lab
  • URL: http://sti-lab.org