| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
OsRac1, a small GTPase in rice, is involved in regulation of NADPH oxidase producing reactive oxygen species during oxidative burst and initiation of cell death during innate immune responses. Recently, we have tried to isolate OsRac1-interacting proteins by various methods. These results revealed the signaling network of OsRac1, named the Defensome network, which is composed of immune receptors. In this study, we isolated MD-2-related lipid recognition proteins as OsRac1 interacting proteins by immunoprecipitation and mass spectrometric analysis. This interaction were found only in constitutively active OsRac1 rice cultured cells, not found in negatively active OsRac1. Our studies revealed that MD-2 protein related cell death and secreted outside of the cell by pathogen stimulus. Fourthermore we found that MD-protein interact with phosphorylation related proteins.
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