Effectors of Fusarium oxysporum f. sp. cepae

• Project/Area Number: 26450057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Yamaguchi University
• Principal Investigator: Ito Shin-ichi 山口大学, 創成科学研究科, 教授 (30243629)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Fusarium oxysporum / エフェクター / 病原性 / 染色体 / タマネギ乾腐病 / ゲノム / Fusariujm oxysporum / タマネギ / ＳＩＸ

Outline of Final Research Achievements

The present study revealed that an effector gene homolog (FocSIX3) of Fusarium oxysporum f. sp. cepae (FOC) is a pathogenicity gene. FocSIX3-specific primers were used for quantitation of FOC in a host plant. FocSIX3 protein is likely processed by a FOC protease in the basal plate tissue of the host; the processed product may participate in a homodimer formation of FocSIX3 protein. A relationship between presence of candidate effector genes and pathogenicity of FOC isolates was observed. Several pathogenicity genes and candidate effector genes are located on the 4 Mb chromosome of FOC.

Research Products

• [Int'l Joint Research] アスワン大学(エジプト)
• [Int'l Joint Research] カルナータカ大学(インド)
• [Journal Article] Detection and quantification of Fusarium oxysporum f. sp. cepae in onion plant. (2015)
  • Author(s): Sasaki, K., Nakahara, K., Shigyo, M., Tanaka, S., Ito, S.
  • Journal Title: Journal of General Plant Pathology Volume: 81 Issue: 3 Pages: 232-236
  • DOI: 10.1007/s10327-015-0589-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic and pathogenic variability of Fusarium oxysporum f. sp. cepae isolated from onion and Welsh onion in Japan. (2015)
  • Author(s): Sasaki, K., Nakahara, K., Tanaka, S., Shigyo, M. and Ito, S.
  • Journal Title: Phytopathology Volume: 105 Issue: 4 Pages: 525-532
  • DOI: 10.1094/phyto-06-14-0164-r
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] タマネギ乾腐病菌の分子生物学的研究とその耐病性育種への応用 (2017)
  • Author(s): 佐々木一紀・伊藤真一
  • Organizer: 平成29年度園芸学会春季大会第20回ネギ類研究小集会
  • Place of Presentation: 日本大学生物資源科学部（神奈川県藤沢市）
  • Invited
• [Presentation] Putative pathogenicity chromosomes of Fuarium oxysporum f. sp. cepae. (2016)
  • Author(s): Sasaki, K. and Ito, S.
  • Organizer: International Joint Seminar of New Core to Core Program A.
  • Place of Presentation: Centara Grand & Bangkok Convention Centre, Central World, Bangkok, Thailand
  • Int'l Joint Research / Invited
• [Presentation] Fusarium oxysporum f. sp. cepaeのエフェクター候補遺伝子の解析 (2016)
  • Author(s): 佐々木一紀・児玉光里・坂根光星・伊藤真一
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 静岡県コンベンションアーツセンター（静岡県静岡市）
• [Presentation] Exploration of candidate genes encoding effector proteins in Fusarium oxysporum f. sp. cepae. (2015)
  • Author(s): Kodama, M. Sasaki, K. and Ito, S.
  • Organizer: 2nd satellite seminar, International Symposium on Microbial Research and Biotechnology for Biomass Utilization
  • Place of Presentation: JR博多シティ（福岡、福岡市）
  • Year and Date: 2015-11-12
  • Int'l Joint Research
• [Presentation] ドラフトゲノム解析によるFusarium oxysporum f. sp. cepaeのエフェクター候補遺伝子の選抜． (2015)
  • Author(s): 児玉光里、佐々木一紀、田中秀平、伊藤真一
  • Organizer: 日本植物病理学会創立100周年記念大会
  • Place of Presentation: 明治大学駿河台キャンパス、東京
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] Genetic and pathogenic diversity of Fusarium oxysporum f. sp. cepae isolated from Allium vegetables in Japan. (2014)
  • Author(s): Ito, S., Sasaki, K.,Tanaka, S
  • Organizer: The 1st Joint Seminar. New Core to Core Program A. Advanced Research Networks on "Establishment of an International Research Core for New Bio-Research Fields with Microbes from Tropical Areas".
  • Place of Presentation: Centara Grand & Bangkok Convention Centre, Central World, Bangkok, Thailand
  • Year and Date: 2014-08-10 – 2014-08-11
• [Presentation] タマネギ乾腐病菌におけるSIX遺伝子ホモログの解析 (2014)
  • Author(s): 佐々木一紀、田中秀平、伊藤真一
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-06-02 – 2014-06-04