Study of behavioural control mechanism throuth the male ejaculation in the sweet potato weevil

• Project/Area Number: 26450067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine (2015-2016); University of the Ryukyus (2014)
• Principal Investigator: KUMANO Norikuni 帯広畜産大学, 畜産学部, 准教授 (90621053)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 配偶行動 / 不妊虫 / 共生細菌 / 微量元素 / ICP-MS / イモゾウムシ / 不妊虫放飼法 / 行動制御

Outline of Final Research Achievements

In this study, the following two points were clarified in the mating behavior of the West Indian sweet potato weevil Euscepes postfasciatus. 1) Both male mating attempt and mating success were higher under high CO2 concentration in air (10,000 ppm) than that in the constant atmosphere (3,000 ppm). 2) The trace element Rubidium in male ejaculation is able to detect quantitatively in inseminated female body by the inductively coupled plasma mass spectrometry (ICP-MS) analyses.

Research Products

• [Journal Article] Symptoms shown by late-stage larvae of the West Indian sweet potato weevil Euscepes postfasciatus (Coleoptera: Curculionidae) infected with Farinocystis sp (2015)
  • Author(s): Tsurui K, Ohishi T, Kumano N, Teruya K, Toyosato T, Shiromoto K.
  • Journal Title: Applied Entomology and Zoology Volume: 50 Issue: 2 Pages: 245-254
  • DOI: 10.1007/s13355-015-0329-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of Long-Term Mass-Rearing on Mating Performance in the West Indian Sweet Potato Weevil, <i>Euscepes postfasciatus</i> (2015)
  • Author(s): 照屋清仁, 熊野了州
  • Journal Title: Japanese Journal of Applied Entomology and Zoology Volume: 59 Issue: 1 Pages: 17-22
  • DOI: 10.1303/jjaez.2015.17
  • NAID: 130005064945
  • ISSN: 0021-4914, 1347-6068
  • Peer Reviewed / Open Access
• [Journal Article] ゾウムシ類におけるオスの性的能力に注目した不妊化技術 (2015)
  • Author(s): 熊野了州
  • Journal Title: 植物防疫 Volume: 6 Pages: 19-23
  • NAID: 40020499444
• [Journal Article] Recent Advances in the Study on Sterile Technique for the West Indian Sweet Potato Weevil, <i>Euscepes postfasciatus</i> (2014)
  • Author(s): 熊野了州
  • Journal Title: Japanese Journal of Applied Entomology and Zoology Volume: 58 Issue: 3 Pages: 217-236
  • DOI: 10.1303/jjaez.2014.217
  • NAID: 130004704265
  • ISSN: 0021-4914, 1347-6068
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Sperm marking using the trace element rubidium to improve the monitoring of the sterile sperm usage in the sterile insect technique (2017)
  • Author(s): Norikuni Kumano, Kiyohito Teruya, Kaori Tsurui, Tetsuya Toyozato
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25
  • Int'l Joint Research
• [Presentation] Sexual conflict may hinder population growth through spatial sexual segregation due to counter-harassment behavior (2017)
  • Author(s): Kaori Tsurui, Tsuyoshi Ohishi, Norikuni Kumano, Kiyohito Teruya, Tetsuya Toyosato and Kazuki Tsuji
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25
  • Int'l Joint Research
• [Presentation] Quantity or quality?: Reproductive development of mass-reared West Indian sweet potato weevil, Euscepes postfasciatus, under high yield conditions for eradication using sterile insect technique (2017)
  • Author(s): Kiyohito Teruya, Tsuyoshi Ohishi, Norikuni Kumano and Kaori Tsurui
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25
  • Int'l Joint Research
• [Presentation] Eradication of the sweetpotato weevil, Cylas formicarius (Coleoptera: Curculionidae), from Kume Island, Okinawa, Japan by using a combination of the sterile insect technique and the male annihilation technique (2017)
  • Author(s): Dai Haraguchi, Takashi Matsuyama, Yasutsune Sadoyama, Tsuguo Kohama, Kunio Kinjo, Norikuni Kumano, Keiko Ohno-Shiromoto and Takashi Kuriwada
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25
  • Int'l Joint Research
• [Presentation] 微量元素ルビジウムを用いた イモゾウムシ不妊オスの 交尾頻度の推定 (2016)
  • Author(s): *熊野了州（帯畜大・昆虫生態）, 照屋清仁（沖縄病害虫防技術セ）, 鶴井香織（琉大・戦略的研究セ）, 豊里哲也（琉球産経）
  • Organizer: 第６０回 応用動物昆虫学会
  • Place of Presentation: 大阪府立大学（大阪府，堺市）
  • Year and Date: 2016-03-27
• [Presentation] 微量元素ルビジウムを用いたイモゾウムシの交尾経験の推定 (2016)
  • Author(s): *熊野了州（帯畜大・昆虫生態）, 照屋清仁（沖縄病害虫防技術セ）, 鶴井香織（琉大・戦略的研究セ）, 豊里哲也（琉球産経）
  • Organizer: 第６３回 日本生態学会 仙台大会
  • Place of Presentation: 仙台国際センター（宮城県，仙台市）
  • Year and Date: 2016-03-20
• [Presentation] イモゾウムシ大量 増殖における歩留りと性成熟の関係 (2015)
  • Author(s): 照屋 清仁 ・大石 毅・熊野 了州・鶴井 香 織
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 原虫に感染したイモゾウムシ体内のグリコーゲン量は減少する (2015)
  • Author(s): 豊里 哲也 ・鶴井 香織・熊野 了州・照屋 清仁・大石 毅
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 天敵は「いる」だけで被食者に影響を与えるか -イリムサーキバラコマユバチの存在がイモゾウムシ次世代数に与える効果 - (2015)
  • Author(s): 鶴井 香織・熊野 了州・原口 大・立田 晴記・豊里 哲也・松山 隆志
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] ナスミバエの実用 的なトラップ形状と誘引剤の検討 (2015)
  • Author(s): 原口 大・安藤 緑樹・江口 恭・西田 律夫・熊野 了州・金城 邦夫・ 佐渡山 安常
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] メスの再交尾を強く制御するイモゾウムシのオスの射精コンディション (2015)
  • Author(s): 熊野 了州・鶴井 香織・豊里 哲也・松山 隆志
  • Organizer: 日本生態学会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2015-03-18 – 2015-03-22
• [Presentation] オスの存在が集団増殖率を下げる行動メカニズム (2015)
  • Author(s): 鶴井香織, 大石毅, 熊野了州,照屋清仁, 豊里哲也
  • Organizer: 日本生態学会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2015-03-18 – 2015-03-22
• [Presentation] 配偶行動を応用する ~南の島のゾウムシ退治~ (2014)
  • Author(s): 熊野了州
  • Organizer: 日本動物行動学会
  • Place of Presentation: 長崎大学
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] 企画シンポジウム「相互作用系の多面的理解から明らかにする個体群生態学」 (2014)
  • Author(s): 熊野了州
  • Organizer: 個体群生態学会
  • Place of Presentation: 筑波大学
  • Year and Date: 2014-10-10 – 2014-10-12
• [Book] Sexual differences in distress sound in the West Indian sweet potato weevil Euscepes postfasciatus. Biological Shape Analysis. The 3rd International Symposium of the Biological Shape Analysis (2015)
  • Author(s): Tatsuta, H., Kuamno, N.
  • Total Pages: 11
  • Publisher: World Scientific, Singapore
• [Book] Morphometric analysis of intraspecific variation of the elytral shape between the wild- and mass-reared sweet potato weevil Cylas formicarius. Biological Shape Analysis. The 3rd International Symposium of the Biological Shape Analysis (2015)
  • Author(s): Kumano, N., Tsurui K., Toyosato, T., and Tatsuta, H.
  • Total Pages: 16
  • Publisher: World Scientific, Singapore