| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
The objective of this study was to characterize the proteineous materials in soil and to elucidate the origin of the proteinous materials. When soils were fractionated by specific gravity and aggregate-size and analyzed by infrared absorption spectroscopy, proteineous materials were mainly present as mineral-associated forms. Alkaline extraction and three-dimensional excitation-emission matrix fluorescence spectroscopy indicated that the proteineous materials in soil were mostly not bound to humic substances. The amounts of proteins extracted during the decomposition process of organic matter in the soil showed a significant positive correlation with the soil microbial biomass. In addition, parts of the extracted proteins were identified as a bacterial extracellular enzyme. These results suggested that a major source of proteineous materials in soil was extracellular proteins produced by soil microorganisms.
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