Studies on the status and origin of proteineous materials in soil
Project/Area Number |
26450072
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant nutrition/Soil science
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Research Institution | Hirosaki University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 土壌有機物 / タンパク質 / 土壌微生物 / 赤外線吸収スペクトル / 三次元蛍光スペクトル / 土壌 / ペプチド態窒素 / 細胞外タンパク質 / 比重分画 / 団粒 / 次亜塩素酸ナトリウム分解 / 土壌窒素 / 赤外分光分析 / 三次元蛍光分析 |
Outline of Final Research Achievements |
The objective of this study was to characterize the proteineous materials in soil and to elucidate the origin of the proteinous materials. When soils were fractionated by specific gravity and aggregate-size and analyzed by infrared absorption spectroscopy, proteineous materials were mainly present as mineral-associated forms. Alkaline extraction and three-dimensional excitation-emission matrix fluorescence spectroscopy indicated that the proteineous materials in soil were mostly not bound to humic substances. The amounts of proteins extracted during the decomposition process of organic matter in the soil showed a significant positive correlation with the soil microbial biomass. In addition, parts of the extracted proteins were identified as a bacterial extracellular enzyme. These results suggested that a major source of proteineous materials in soil was extracellular proteins produced by soil microorganisms.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Changes in the soil C and N contents, C decomposition and N mineralization potentials in a rice paddy after long-term application of inorganic fertilizers and organic matter.2016
Author(s)
Cheng, W., Padre, A.T., Sato, C., Shiono, H., Hattori, S., Kajihara, A., Aoyama, M., Tawaraya, K. and Kumagai, K.
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Journal Title
Soil Science and Plant Nutrition
Volume: 62
Issue: 2
Pages: 1-8
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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