| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The Calvin-Benson cycle enzymes Rubisco and transketolase were co-overproduced in rice in order to enhance photosynthetic performance and plant growth. The mRNA levels of these genes were increased in transgenic plants with their co-overexpression. The protein levels of Rubisco and transketolase on a leaf area basis were increased by 35 to 53% and 39 to 84%, respectively. However, the rates of CO2 assimilation were similar to those in wild-type plants. Thus, co-overproduction of Rubisco and transketolase did not lead to enhancement of photosynthetic performance via improvement of the Calvin-Benson cycle metabolism in rice.
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