| Project/Area Number |
26450075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tanaka Mayuki 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (80546292)
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| Research Collaborator |
Shirouzu Mikako 理化学研究所, タンパク質機能・構造研究チーム, チームリーダー (70280732)
Yokoyama Takeshi 理化学研究所, タンパク質機能・構造研究チーム, 研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | シロイヌナズナ / ホウ素 / リボソーム / 輸送体 / uORF / 上流ORF / mRNA分解 / 転写後制御 |
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| Outline of Final Research Achievements |
Purpose of this study is to understand the boron sensing mechanisms of ribosome-mRNA complex stalled at the minimum open reading frame, AUGUAA.
First, in order to identify genes involved in B-dependent ribosome stalling at AUGUAA accompanied by mRNA degradation, I screened mutant plants from a methanesulfonate-mutagenized population by GFP fluorescence as an indicator and performed next generation sequencer and dCaps marker analysis. Then, I identified causal genes of these mutants plants. Second,I successfully obtained 3D structures of ribosome-mRNA complex by Cryo-electron microscopy.
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