Analysis of sensing mechanisms of mineral nutrient through ribosome-RNA complex
Project/Area Number |
26450075
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant nutrition/Soil science
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Research Institution | The University of Tokyo |
Principal Investigator |
Tanaka Mayuki 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (80546292)
|
Research Collaborator |
Shirouzu Mikako 理化学研究所, タンパク質機能・構造研究チーム, チームリーダー (70280732)
Yokoyama Takeshi 理化学研究所, タンパク質機能・構造研究チーム, 研究員
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | シロイヌナズナ / ホウ素 / リボソーム / 輸送体 / uORF / 上流ORF / mRNA分解 / 転写後制御 |
Outline of Final Research Achievements |
Purpose of this study is to understand the boron sensing mechanisms of ribosome-mRNA complex stalled at the minimum open reading frame, AUGUAA. First, in order to identify genes involved in B-dependent ribosome stalling at AUGUAA accompanied by mRNA degradation, I screened mutant plants from a methanesulfonate-mutagenized population by GFP fluorescence as an indicator and performed next generation sequencer and dCaps marker analysis. Then, I identified causal genes of these mutants plants. Second,I successfully obtained 3D structures of ribosome-mRNA complex by Cryo-electron microscopy.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] The Minimum Open Reading Frame, AUG-Stop, Induces Boron-Dependent Ribosome Stalling and mRNA Degradation.2016
Author(s)
Tanaka, M., Sotta, N., Yamazumi, Y., Yamashita, Y., Miwa, K., Murota, K., Chiba, Y., Hirai, MY., Akiyama, T., Onouchi, H., Naito, S. and Fujiwara, T
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Journal Title
The Plant Cell
Volume: 28
Issue: 11
Pages: 2830-2849
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] OsNIP3;1, a rice boric acid channel, regulates boron distribution and is essential for growth under boron-deficient conditions.2014
Author(s)
Hanaoka, H., Uraguchi, S., Takano, J., Tanaka, M. and Fujiwara, T.
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Journal Title
The Plant Journal
Volume: 78
Issue: 5
Pages: 890-902
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparison of BOR1-like gene expression in two genotypes with different boron efficiencies in commercial crop plants in Thailand2014
Author(s)
Leaungthitikanchana, S., Tanaka, M., Lordkaew, S., Jamjod, S., Rerkasem, B. and Fujiwara, T.
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Journal Title
Soil Science and Plant Nutrition
Volume: 60
Issue: 3
Pages: 333-340
DOI
Related Report
Peer Reviewed / Open Access
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