Histochemical and cytochemical approaches toward elucidating the mechanisms of functional expression of barley high-affinity nitrate transport system
Project/Area Number |
26450076
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant nutrition/Soil science
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Research Institution | Niigata University |
Principal Investigator |
Sueyoshi Kuni 新潟大学, 自然科学系, 教授 (10216278)
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Research Collaborator |
野澤 大樹
高田 雅大
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 硝酸イオン / トランスポーター / オオムギ / 硝酸吸収 / 輸送タンパク質 / 硝酸 / 細胞内局在性 / 高親和性硝酸輸送系 |
Outline of Final Research Achievements |
In the present study, an interaction between two proteins (HvNRT2.1 and HvNAR2.3), both of which configure the high-affinity nitrate transport system in barley roots, was investigated histochemically and cytochemically. These two proteins localize on the plasma membrane of the outermost cell layer in the barley root cortex. In addition, these proteins interact on the plasma membrane as revealed by BiFC analysis. When HvNRT2.1 and HvNAR2.3 were expressed individually in epidermal cells of onion, each protein failed to localize the plasma membrane. These results indicate that these two proteins may need each other for localizing on the plasma membrane.
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Report
(5 results)
Research Products
(7 results)
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[Presentation] Ammonium transporter, HvAMT1, in barley2015
Author(s)
Masato Shinohara, Shinji Ishikawa, Norikuni Ohtake, Takuji Ohyama and Kuni Sueyoshi
Organizer
The 5th Asian Conference on Green Technology in Agriculture
Place of Presentation
Chiang Mai, Thailand
Year and Date
2015-07-20
Related Report
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