| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In the present study, an interaction between two proteins (HvNRT2.1 and HvNAR2.3), both of which configure the high-affinity nitrate transport system in barley roots, was investigated histochemically and cytochemically. These two proteins localize on the plasma membrane of the outermost cell layer in the barley root cortex. In addition, these proteins interact on the plasma membrane as revealed by BiFC analysis. When HvNRT2.1 and HvNAR2.3 were expressed individually in epidermal cells of onion, each protein failed to localize the plasma membrane. These results indicate that these two proteins may need each other for localizing on the plasma membrane.
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